Thermo Scientific Evolution 200 Series User Manual page 185

Thermo Scientific
Feature
Name
Description (optional)
Type
Dye 1 and Dye 2
Pathlength
Related Topics
Measurement Tab for Proteins and Labels
Instrument Tab for Proteins and Labels
Accessories Tab for Proteins and Labels
Samples Tab for Proteins and Labels
Description
Application name.
Description of the template.
Type of analysis to perform.
1 Abs = 1 mg / mL is a general reference setting based on a 0.1%
(1 mg/ml) protein solution. It produces an absorbance of 1.0 A at
280 nm (where the pathlength is 10 mm, or 1 cm).
BSA is a Bovine Serum Albumin reference. Protein concentrations
are calculated using the mass extinction coefficient of 6.7 at
280 nm for a 1% (10 mg/ml) BSA solution.
IgG is an IgG reference. Protein concentrations are calculated
using the mass extinction coefficient of 13.7 at 280 nm for a 1%
(10 mg/ml) IgG solution.
For Lysozyme, protein concentrations are calculated using the
mass extinction coefficient of 26.4 at 280 nm for a 1%
(10 mg/ml) Lysozyme solution.
Other protein (ε & MW) is the entered molar extinction
-1 -1
coefficient (M cm ) and molecular weight (MW) in kilodaltons
(kDa) for the respective protein reference. The maximum values
are 99999 X 1000 for ε and 9999 X 1000 for MW
Other protein (ε 1%) is the mass extinction coefficient
-1 -1
(l g cm ) for a 10 mg/ml (1%) solution of the respective
reference protein.
Fluorescent dyes whose absorbance will be used in concentration
correction calculations.
Distance the light travels through the sample.
4
Life Science Applications
Proteins and Labels
Evolution 200 Series User Guide 171