Chapter 2 Before You Begin; Precautions; Avoid Nucleic Acid Contamination; Avoid Chip Damage - Thermo Scientific Ion 520 User Manual

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Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

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Guidelines for using Ion Chef
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Prepare the Ion Chef
Precautions

Avoid nucleic acid contamination

IMPORTANT! A primary source of contamination is spurious DNA fragments from previous sample
processing steps. Do not introduce amplified DNA into the library preparation laboratory or work area.

Avoid chip damage

IMPORTANT! To avoid possible damage to the chip due to electrostatic discharge, ground yourself
before picking up a chip or placing a chip on a surface such as a lab bench. For example, touch the
metal trim on the chip compartment before inserting or removing a chip from the chip clamp.

Protection by equipment

WARNING! The protection that is provided by the equipment can be impaired if the instrument is
operated outside the environment and use specifications, the user provides inadequate maintenance,
or the equipment is used in a manner that is not specified by the manufacturer (Thermo Fisher
Scientific).
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consumables and reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
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Instrument for use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Before you begin
Ion 520 ™ & Ion 530 ™ ExT Kit − Chef User Guide