Overview; Nucleic Acid Concentration Calculations - Thermo Scientific Evolution 200 Series User Manual

Nucleic Acid Concentration Calculations

Nucleic Acid Purity Measurements
Thermo Scientific
Related Topics
Nucleic Acid Concentration Calculations
Application Settings
Unique Screen Features
Making Nucleic Acid Measurements
Oligo Calculator
The Nucleic Acid application is used for determining the concentration and purity of nucleic
acid samples. The nucleic acid concentration is determined by multiplying the absorbance at
260 nm by a factor (default, 50). This factor is essentially the extinction coefficient in units of
ng-cm/ml. Using this coefficient gives the manipulated equation
ε
l
c = (A * )/
where c is the nucleic acid concentration in ng/microliter, A is the absorbance in AU, ε is the
wavelength-dependent extinction coefficient in ng-cm/microliter, and
centimeters.
The generally accepted extinction coefficients for nucleic acids are:
Coefficient
Double-stranded DNA 50 ng-cm/μl
Single-stranded DNA
RNA
Related Topics

Overview

Application Settings
Unique Screen Features
Making Nucleic Acid Measurements
Oligo Calculator
The 260/280 nm ratio assay determines the purity of nucleic acids based on the 260/280
ratio.
Value
33 ng-cm/μl
40 ng-cm/μl
4
Life Science Applications
Nucleic Acid
is the pathlength in
Evolution 200 Series User Guide 139