Bradford Standard Curves; Making Protein Bradford Assay Measurements - Thermo Scientific Evolution 200 Series User Manual

Bradford Standard Curves

Making Protein Bradford Assay Measurements

Thermo Scientific
Related Topics
Type Tab for Protein Bradford
Measurement Tab for Protein Bradford
Standards Tab for Protein Bradford
Instrument Tab for Protein Bradford
Accessories Tab for Protein Bradford
A standard curve is required for colorimetric protein analysis.
• A standard curve can be generated using two or more standards.
• The multipoint curve capability allows multiple replicates for each standards. Standards
can be run in any order; however, best practice dictates that the standards be measured
from the lowest concentration to the highest.
• Determine whether or not to use a standard when analyzing samples against the standard
curve.
• Establishing a new standard curve requires creating a new workbook.
• If a previously saved workbook is used, all concentration calculations for newly measured
samples will be based on the standard absorbance values saved in the workbook.
Note Each workbook will archive only one standard curve.
Related Topics
Overview
Application Settings
Bradford Standard Curves
Making Protein Bradford Assay Measurements
See Sample and Standards Preparation for more information.
Evolution 200 Series User Guide
4
Life Science Applications
Protein Bradford
197