Nucleic Acid - Thermo Scientific Evolution 200 Series User Manual

4
Life Science Applications

Nucleic Acid

Method
Nucleic Acid
Nucleic Acid
Labels
Protein A280
Proteins & Labels
Pierce BCA
Protein Bradford
Pierce Modified
Lowry
Pierce 660 nm
Protein Assay
Protein Biuret
Nucleic Acid
Overview
138 Evolution 200 Series User Guide
Analytical
Reference
Wavelength
Wavelength
(Quant)
340
340
340
340
562 340
595 340
750 405
660 340
550 340
Contents
• Overview
• Nucleic Acid Concentration Calculations
• Application Settings
• Unique Screen Features
• Making Nucleic Acid Measurements
• Oligo Calculator
See Unique Screen Features
Nucleic Acid Concentration Calculations
Start Stop
Bandwidth
(Scan) (Scan)
220 350 1.0
220 850 1.0
220 850 1.0
220 850 1.0
750 450 1.0
750 450 1.0
900 600 1.0
850 550 1.0
750 450 1.0
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Use the Nucleic Acid application to measure nucleic acid
samples for concentration and purity.
for information about the results the application calculates. See
for information about the calculations.
Integration Data
Time (sec) Interval
(Fixed, Scan) (Scan)
1.0, 0.08 0.50
1.0, 0.16 1.00
1.0, 0.08 0.5
1.0, 0.16 1.0
1.0, 0.12 1.00
1.0, 0.12 1.00
1.5, 0.12 1.00
1.5, 0.12 1.00
1.0, 0.12 1.00
Scan
Speed
(nm/min)
375
375
375
375
500
500
500
500
500
Thermo Scientific