Thermo Scientific Ion 520 Chip Kit Manuals

Thermo Scientific Ion 520 Chip Kit Manuals

Manuals and User Guides for Thermo Scientific Ion 520 Chip Kit. We have 1 Thermo Scientific Ion 520 Chip Kit manual available for free PDF download: User Manual

Thermo Scientific Ion 520 User Manual

Thermo Scientific Ion 520 User Manual (78 pages)

Brand: Thermo Scientific | Category: Laboratory Equipment | Size: 2 MB

Table of Contents

Table of Contents
3
CHAPTER 1 Product Information

6
CHAPTER 2 Before You Begin

14
CHAPTER 3 Create a Planned Run

17
- About Planned Runs
  17
- Create a Planned Run
  18
- Planned Run Workflow Key Fields
  22
- Start the Ion Chef
  24
- Materials Required
  24
- Dilute the Library
  24
- Guidelines for Library Dilution
  25
- Dilute the Sample Library
  25
- Dilute the Control Library
  25
- Prepare the Consumables
  25
- Add the Diluted Libraries to the Ion Chef
  26
- Library Sample Tube
  26
- System
  27
- Load the Ion Chef
  27
- Load the Tip Racks and PCR Plate
  28
- Load the Reagents and Solutions Cartridges
  29
- Load the Recovery Tubes and Enrichment Cartridge V2
  31
- Load the Chip-Loading Centrifuge
  33
- Confirm that Consumables Are Correctly Installed
  35
- Pre-Run Checklist
  36
- Run
  37
- Start the Ion Chef
  37
- Unload the Chip for Sequencing
  42
- Initialize the Sequencer
  45
- Ion Genestudio ™ S5 Systems Component Positions
  45
- Before You Begin
  46
- When a Manual Cleaning of the Sequencer Is Required
  46
- Initialize the Sequencer
  47
CHAPTER 6 Start the Sequencing Run

49
- Chip Handling Guidelines
  49
- Start the Sequencing Run
  49
- Maintain the Sequencer
  52
- Materials Required
  52
- Clean or Decontaminate the Sequencer
  52
- Reagent Consumables Disposal
  52
- Scrubber Removal and Disposal
  52
- Recycle Ion S5
  53
- Ext Wash Solution and Ion S5
  53
- Cleaning Solution Bottles
  53
- About the Cleaning Protocol
  54
- Materials Required
  54
- Instrument
  54
- System
  54
- Remove and Dispose of Used Consumables
  55
- Inspect and Clean the Recovery Centrifuges and Buckets
  57
- Start the Cleaning
  58
APPENDIX A Troubleshooting

60
APPENDIX B Supplemental Procedures

65
APPENDIX C Safety

68
- Symbols on this Instrument
  68
- Conformity Symbols
  69
- Location of Safety Labels on this Instrument
  70
- Safety Information for Instruments Not Manufactured by Thermo Fisher Scientific
  70
- Instrument Safety
  70
- General
  70
- Physical Injury
  70
- Electrical Safety
  71
- Cleaning and Decontamination
  71
- Ultraviolet (UV) Safety
  71
- Safety and Electromagnetic Compatibility (EMC) Standards
  71
- Safety Compliance
  72
- Emc
  72
- Environmental Design
  72
- Instrument Safety - Ion S5
  73
- Ion Genestudio ™ S5 Systems
  73
- Ion Genestudio S5 Systems
  73
- Chemical Safety
  73
- Biological Hazard Safety
  74
- Documentation and Support
  75
- Related Documentation
  75
- Customer and Technical Support
  76
- Limited Product Warranty
  76

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