Thermo Scientific Evolution 200 Series User Manual page 101

Ratioing Two Scan Spectra
Thermo Scientific
Use Ratio in the Math menu to divide a scan spectrum by another.
To ratio two scan spectra
1. Choose Math (menu) > Ratio.
The selected spectrum appears in the Operand 1 pane. To use a different listed spectrum,
select it and click Add in the Operand 1 box.
The list includes only current workbook spectra. To add others:
a. Click Browse.
b. Select a folder in the left pane (use the features above it to navigate), a workbook in
the middle pane, and spectra in the upper-right pane. To select multiple spectra, hold
down Shift or Ctrl. Selected spectra appear in the lower-right pane.
c. Click OK. Each added spectrum is listed with asterisks at the beginning of its name
to indicate the data is not in the current workbook.
2. Select the spectrum by which to divide the first and click Add in the Operand 2 box.
3. To multiply the second spectrum by a factor other than 1, drag the slider or use Factor.
Type a value in the Factor text box or use its up and down arrow buttons. Use Increment
to set the amount of change these buttons make to the factor when clicked. For example,
with Factor set to 1.000 and Increment set to 0.500, clicking the Factor up arrow button
changes the factor to 1.500.
4. To perform a math operation on the division result, click Operate on Result and choose
an operation. Otherwise, go to the next step.
Note To use the result in another Ratio operation, use Workbook or Temporary to
add it to the list (see step 5), click Add in the Operand 1 box, and continue with step
2.
Perform the operation as explained in the appropriate related topic below.
To return to the previous window, click Back.
To perform additional math operations, repeat this step.
5. To save the result in the current workbook, click Workbook and name the spectrum
when prompted.
The data is added to the list and is available for operations.
To add the result to the list without saving it, click Temporary. Asterisks at the beginning
of a spectrum name indicate the data is not saved.
2
INSIGHT Software
Operation
Evolution 200 Series User Guide 87