Making Nucleic Acid Labels Measurements - Thermo Scientific Evolution 200 Series User Manual

Making Nucleic Acid Labels Measurements

Thermo Scientific
While performing this procedure,
spectral display.
To make a Nucleic Acid Labels measurement
1. Click Measure Nucleic Acid Labels in
Note To start an analysis immediately after viewing or changing the template settings,
click the Measure action button instead and skip to step 3.
2. Click Measure.
3. Follow the instructions that appear.
Use the appropriate buffer for the blank, generally the same buffer used to prepare or
dissolve the sample. The buffer should be the same pH and of a similar ionic strength as
the sample solution.
4. When samples information appears, modify it if desired.
More:
If Sample averaging on the Samples tab in Settings was set to Duplicate, "D" at the end of
a sample name indicates the second measurement to be made of the sample. If Sample
averaging was set to Triplicate, "D" and "T" at the end of sample names indicate the
second and third measurements to be made, respectively.
To enter previously saved samples information, use Load Samples. To save the samples
information, use Save Samples.
5. If only one sample will be measured, insert it.
6. Click Continue.
7. Follow any instructions that appear, such as to install a specified sample.
Note Use a fresh aliquot of sample for each measurement.
The Run Chart tab plots the concentration of the measured component versus sample
number. If Use concentration limits was selected on the Samples tab, horizontal limit
lines show whether the concentrations are within the specified limits:
click here for information about features to the right of the
Nucleic Acid Labels.
4
Life Science Applications
Nucleic Acid Labels
Evolution 200 Series User Guide 157