Introduction To Small Volume Measurements - Thermo Scientific Evolution 200 Series User Manual

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nanoCell

Introduction to Small Volume Measurements

282 Evolution 200 Series User Guide
Figure 1. Light path of the nanoCell accessory
Related Topics
Using the nanoCell
Cleaning the nanoCell
Pathlength Correction Factors
Frequently Asked Questions
Small volume samples are measured by reducing the measurement pathlength to
accommodate the reduced volume. For example, it would be impossible to measure a 1 μl
drop of solution in a standard 1 cm cuvette.
To make small volume measurements, the pathlength of the nanoCell accessory is decreased
by a factor of 10 (1.0 mm pathlength) or 50 (0.2 mm pathlength) compared to a traditional
10 mm pathlength cuvette.
The Beer Lambert law relates the measured absorbance of a solution to the concentration of
that solution. The Beer Lambert equation is:
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