Troubleshooting - Thermo Scientific Evolution 200 Series User Manual

28
Smart Sipper

Troubleshooting

428 Evolution 200 Series User Guide
Problem Possible Cause
Pump does not The accessory is not
operate installed properly or
the wrong pump
tubing is used.
Pump does not Pump tubing is
draw crimped or damaged
Noise peaks in There are air bubbles
the spectral data in the flow cell
or inconsistent
results
Solution
Remove and reinstall the accessory, install the
Sipper front panel and connect the data cable. See
Installation.
Ensure the pump tubing is the correct diameter
and material. See Pump Tubing Specifications
and Pump Tubing Chemical
Check the entire pumping system for damage or
excessive wear and replace sections as necessary.
See Installing Pump Tubing
Cell.
To prevent crimping, remove pump tubing before
storing the accessory. When the pump is idle,
leave the instrument and accessory powered on
and some liquid in the sample intake container.
The accessory periodically "sips" a small volume
to redistribute the pressure on the tubing.
Ensure the tubing is compatible with your sample
material. Many factors (temperature, length of
service, chemical concentration, etc.) can affect
chemical resistance; test the tubing under your
conditions. See Pump Tubing Chemical
Compatibility.
Rinse flow cells and tubing thoroughly after use.
See Cleaning Flow Cells.
Inspect the flow cell for bubbles, which diffract
the light so less energy is passed to the detector.
To prevent air bubbles from entering the cell, use
sufficient volume so the sample material fills at
least 8 cm of the inlet tube in front of the flow
cell. (Use an air gap if the sample material is
limited.) See Operation.
To remove air bubbles adhering to flow cell walls
(occurs frequently with biological materials),
remove the cell and soak it in a recommended
detergent. See Cleaning Flow
finished, reconnect the cell and flush it
thoroughly with laboratory grade purified water.
Compatibility.
and Installing a Flow
Cells. When
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