Thermo Scientific Evolution 200 Series User Manual page 177

Thermo Scientific
Feature Description
Analysis wavelength Wavelength(s) to use for the analysis.
Correction Specifies bichromatic normalization of the absorbance data.
To use uncorrected data, select None. Without baseline correction,
spectra may be offset from the baseline. If this offset is significant, the
calculated protein concentration may be higher than the true value.
Single point subtracts the sample absorbance at the entered
wavelength from the absorbance at every data point.
Sloping baseline draws a line between the data points at the entered
wavelengths, finds its absorbance value at the analytical wavelength,
and subtracts it from the measured absorbance.
Component name Component to quantify.
4
Life Science Applications
Protein A280
Evolution 200 Series User Guide 163