Working With Standard Curves; Making Pierce Bca Measurements - Thermo Scientific Evolution 200 Series User Manual

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Life Science Applications
Pierce BCA

Working With Standard Curves

Making Pierce BCA Measurements

Sample and Standards Preparation
186 Evolution 200 Series User Guide
Related Topics
Type Tab for Pierce BCA
Measurement Tab for Pierce BCA
Standards Tab for Pierce BCA
Instrument Tab for Pierce BCA
Accessories Tab for Pierce BCA
A standard curve is required for colorimetric protein analysis.
• A standard curve can be generated using two or more standards.
• The multipoint curve capability allows multiple replicates for each standards. Standards
can be run in any order; however, best practice dictates that the standards be measured
from the lowest concentration to the highest.
• Determine whether or not to use a standard when analyzing samples against the standard
curve.
• Establishing a new standard curve requires creating a new workbook.
• If a previously saved workbook is used, all concentration calculations for newly measured
samples will be based on the standard absorbance values saved in the workbook.
Note Each workbook will archive only one standard curve.
Related Topics
Overview
Application Settings
Making Pierce BCA Measurements
• See the manufacturers' guidelines and recommendations for standard and sample
preparation.
• Prepare both standards and unknowns in the same manner. Use a diluent of the same pH
and ionic strength for all blanks, standards and unknown samples.
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