Thermo Scientific Evolution 200 Series User Manual page 191

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2. Click Measure.
3. Follow the instructions that appear.
Use the appropriate buffer for the blank, generally the same buffer used to prepare or
dissolve the sample. This solution should be the same pH and of a similar ionic strength
as the sample solution.
4. When samples information appears, modify it if desired.
More:
If Sample averaging on the Samples tab in Settings was set to Duplicate, "D" at the end of
a sample name indicates the second measurement to be made of the sample. If Sample
averaging was set to Triplicate, "D" and "T" at the end of sample names indicate the
second and third measurements to be made, respectively.
To enter previously saved samples information, use Load Samples. To save the samples
information, use Save Samples.
5. If only one sample will be measured, insert it.
6. Click Continue.
7. Follow any instructions that appear, such as to install a specified sample.
The Run Chart tab plots the concentration of the measured component versus sample
number. If Use concentration limits was selected on the Samples tab, horizontal limit
lines show whether the concentrations are within the specified limits:
To copy this plot, right-click it and choose Copy to Clipboard.
4
Life Science Applications
Proteins and Labels
Evolution 200 Series User Guide 177