Protein Bradford; Overview; Bradford Reagent Kits And Protocols; Application Settings - Thermo Scientific Evolution 200 Series User Manual

Bradford Reagent Kits and Protocols

Application Settings

Thermo Scientific
This assay is commonly used for determining protein concentration. It is often used for more
dilute protein solutions where lower detection sensitivity is needed and/or in the presence of
components that also have significant UV (280 nm) absorbance. Like the other colorimetric
assays, the Bradford assay requires generating a standard curve before measuring sample
proteins.
The Bradford procedure uses the protein-induced absorbance shift of Coomassie Blue dye to
595 nm to measure protein concentration. The bound protein-dye complex is measured at
595 nm and normalized at 750 nm. A single stabilized reagent mixture containing Coomassie
Blue dye, alcohol, and surfactant in kit form is available from numerous manufacturers.
Related Topics
Application Settings
Bradford Standard Curves
Making Protein Bradford Assay Measurements
Follow the assay kit manufacturer's recommendations for all standards and samples
(unknowns), ensuring they are subjected to the same timing and temperature throughout the
assay.
Protein standards (BSA) for generating a standard curve may also be provided by the Bradford
assay manufacturer.

Type Tab for Protein Bradford

Measurement Tab for Protein Bradford

Standards Tab for Protein Bradford

Instrument Tab for Protein Bradford

Accessories Tab for Protein Bradford

Samples Tab for Protein Bradford

Related Topics

Overview

Bradford Standard Curves
Making Protein Bradford Assay Measurements
To set data acquisition parameters for a
workbook, click Settings to access:
4
Life Science Applications

Protein Bradford

Protein Bradford
Evolution 200 Series User Guide 191