Pierce 660 Nm Protein Assay - Thermo Scientific Evolution 200 Series User Manual

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Life Science Applications

Pierce 660 nm Protein Assay

Pierce 660 nm Protein Assay
Overview
212 Evolution 200 Series User Guide
To measure a sample again, right-click its row in the results table and choose Remeasure.
After the remeasurement, the previous information for the sample is crossed out (but not
removed from the table).
To copy the data on the Standard Curve(s) tab or Run Chart tab, right-click the plot and
choose Copy to Clipboard.
Related Topics
Overview
Application Settings
Lowry Standard Curves
Data Display
Contents
• Overview
• Application Settings
• Pierce 660 nm Assay Workbooks
• Standard Curves
• Making Pierce 660 nm Protein Assay Measurements
The proprietary dye-metal complex binds to protein in acidic conditions, causing a shift in
the dye's absorption maximum, which is measured at 660 nm. The complex is reddish-brown
and turns green upon protein binding. This color change is produced by deprotonation of the
dye at low pH facilitated by interactions with positively charged amino acid groups in the
protein. Consequently, the complex interacts primarily with basic residues in the protein, such
as histidine, arginine and lysine, and to a lesser extent tyrosine, tryptophan and phenylalanine.
The Thermo Scientific Pierce 660 nm Protein Assay
reagent is a ready-to-use formulation that offers rapid,
accurate and reproducible colorimetric detection of
minute amounts of protein in solution. The reagent is
ideal for measuring total protein concentration in samples
containing reducing agents and/or detergents.
Thermo Scientific