Chapter Overview; Chapter 2 Design The Standard Curve Experiment - Applied Biosystems 7500 Getting Started

Chapter 2 Design the Standard Curve Experiment

Chapter Overview

Chapter Overview

Example
Experiment
Workflow
Notes
18
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
This chapter explains how to use the Design Wizard in the 7500 software to set up the
standard curve example experiment. The Design Wizard guides you through Applied
Biosystems recommended best practices as you enter design parameters for the example
experiment.
When you design your own experiments, you can select alternate workflows (see
Note:
"Using This Guide with Your Own Experiments" on page
1. Create a new experiment.
2. Define the experiment properties.
3. Define the methods and materials.
4. Set up the targets.
5. Set up the standards.
6. Set up the samples.
7. Set up the run method.
8. Review the reaction setup.
9. Order materials for the experiment.
10. Finish the Design Wizard.
Start Experiment
Design the Experiment (Chapter 2)
Prepare the Reactions (Chapter 3)
Run the Experiment (Chapter 4)
Analyze the Experiment (Chapter 5)
End Experiment
11).
Experiments