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Calibration overview
Calibration and
maintenance
schedule
QuantStudio
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
™
™
The QuantStudio
6 and 7 Flex Systems require regular calibration and maintenance
for proper operation. To ensure proper operation of your instrument, perform weekly,
monthly, and semiannual maintenance as indicated in the following table.
IMPORTANT! Perform calibrations and run experiments under the environmental
conditions specified in Appendix H, "Instrument specification and layout". Exposure
to extreme temperatures can have adverse effects on the run results, as well as
shortening the life span of the instrument components.
IMPORTANT! Do not use organic solvents to clean the QuantStudio
Systems.
Frequency
Check the computer disk space. If necessary, archive or back up
your experiment files and instrument settings.
Weekly
Power off the computer that controls the instrument, then power
on the computer after 30 seconds.
Clean the surface of the instrument with a lint-free cloth.
Perform a background calibration.
Monthly
Run disk cleanup and disk defragmentation.
Perform an instrument self test.
Perform an ROI calibration.
Perform a background calibration.
Semi-annually (every
Perform a uniformity calibration.
6 months)
Perform a dye calibration.
(TaqMan
calibration.
Perform an RNase P instrument verification run.
As needed
Replace the instrument lamp.
[1]
You can perform a background calibration to check for contamination.
[2]
A normalization calibration is not required for the 96-well plate blocks (0.1-mL or 0.2-mL) or the 384-well
plate block.
[3]
After replacing the instrument lamp, perform all calibrations and an RNase P instrument verification run.
Chapter 2 Calibrate the instrument
User-performed maintenance task
Array Card block only) Perform a normalization
®
[2]
[3]
Calibration overview
™
6 and 7 Flex
[1]
[1]
2
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