Preparing Plates and Performing the Pure Dye Calibration
Selecting the Dye
In the Pure Spectra Calibration Manager:
1.
In the Dye List field, select a pure dye to
a.
calibrate.
Click
b.
If you are prompted to disconnect the plate
c.
document, click
A message prompts you to load the plate.
d.
Do not click Yes or No at this time.
Preparing and Loading a Pure Dye Plate
Remove the appropriate pure dye plate from its
1.
packaging.
Do not discard the packaging for
IMPORTANT!
the pure dye plate. The pure dye plate can be
used up to three times if it is stored in its original
packaging sleeve.
Standard plates only: Vortex the plate for
2.
5 seconds. Do not vortex Fast plates.
(Remaining steps apply to both standard and Fast
plates.)
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
.
.
Chapter 6 Performing the Pure Dye Calibration
Preparing Plates and Performing the Pure Dye Calibration
a
b
Click
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