Guidelines For Preparing Calibration Plates And Array Cards - Applied Biosystems QuantStudio 6 Flex User Manual

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Chapter 2 Calibrate the instrument

Guidelines for preparing calibration plates and array cards

Figure 4 Calibration reminders screen for the instrument with a TaqMan

Guidelines for preparing calibration plates and array cards

36
Observe the following guidelines when preparing calibration plates and array cards:
• Wear appropriate protective eyewear, clothing, and gloves.
• Prepare and run calibration plates and array cards within the recommended time
limits listed below.
Consumable
All 96-/384-well calibration consumables
All array card calibration consumabes
RNase P plate (96-/384-well)
RNase P array card
[1]
All 96-well plates and 384-well plates used in the calibration of the instrument (ROI, background,
uniformity, and dye).
[2]
All array cards used in the calibration of the QuantStudio
uniformity, dye, and normalization).
[3]
For use with the QuantStudio
• (Plates only) Do not discard the packaging for the calibration plates. Each plate
can be used to calibrate the QuantStudio
6 months if it is stored in its packing sleeve.
QuantStudio 6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
™
®
Array Card block
[1]
[2]
[3]
™ 7 Flex System only.
™ 6 and 7 Flex Systems 3 times for up to
After thawed,
Time to thaw
run within...
30 minutes 120 minutes
30 minutes 120 minutes
5 minutes 30 minutes
15 minutes 60 minutes
™ 7 Flex System only (ROI, background,