Scanning Plates - Applied Biosystems CytoFluor 4000 Series User Manual

Fluorescence multi-well plate reader
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Chapter 3
Scanning Operations
3

3.6 Scanning Plates

Before scanning
3-24
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1.
Set up the run as described in the previous sections.
Check that the plate carrier is outside the measurement
chamber and in the load position. If it's not, press the
Plate Out Button on the main setup screen. (This
button toggles between Plate Out and Plate In). You
can also access Plate Out from the Maintenance
screen (under the Maintenance menu).
It is safer to give the file a name before you start a
scan. If the file has a name, the system saves each
cycle to the file as it completes the cycle. If you did not
name the file, you need to save it through the Save
As... command under File at the completion of the run.
Otherwise, you could lose the data if something
disturbed the run (for example, a power failure).
2. Warm up the scanner lamp for a minimum of 10 minutes
prior to your first scan. You can turn the lamp on by
clicking the Lamp On button, if necessary, before setting
up the scan parameters.
3. Place your prepared plate into the plate carrier. Make sure
the plate is fully seated in the carrier and well A1 is at the
top left (towards the back of the scanner) as shown:
Figure 3-8 Seating the Plate in the Carrier
CAUTION
A1

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