Measure Uv-Vis; To Make Uv-Vis Measurements - Thermo Scientific NanoDrop OneC User Manual

3
Applications

Measure UV-Vis

Measure UV-Vis
Measure UV-Vis

To make UV-Vis measurements

C
12 NanoDrop One with NanoDropQC Software User Guide
Measures the absorbance of any sample at
up to 40 wavelengths across the ultra-violet
(UV) and visible regions of the spectrum.
Measure UV-Vis
Reported Results
Settings
Detection Limits
The UV-Vis application allows the instrument to function as a conventional
spectrophotometer. Sample absorbance is displayed on the screen from 190 nm to 850 nm.
Up to 40 wavelengths can be designated for absorbance monitoring and inclusion in the
report. Automatic pathlength adjustment and a single-point baseline correction can also be
used.
NOTICE
• Do not use a squirt or spray bottle on or near the instrument as liquids will flow into
the instrument and may cause permanent damage.
• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will permanently
damage the quartz fiber optic cables.
Before you begin...
Before taking pedestal measurements with the NanoDrop One instrument, lift the instrument
arm and clean the upper and lower pedestals. At a minimum, wipe the pedestals with a new
laboratory wipe. For more information, see
To measure a sample using the UV-Vis application
1. From the Home screen, select UV-Vis.
2. Specify up to 40 wavelengths to monitor
whether automated pathlength adjustment, analysis wavelength, and baseline correction
will be used.
Cleaning the Pedestals.
(or you can specify them later if desired) and
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