Working With A Magnetic Rod; Collecting Magnetic Particles; Releasing Magnetic Particles; Washing Magnetic Particles - Thermo Scientific KingFisher Flex User Manual

Functional Description

Working with a magnetic rod

Working with a
magnetic rod
Collecting magnetic
Releasing magnetic
Washing magnetic

Incubation

Changing the volume
during the magnetic
particle processing
18 Thermo Scientific KingFisher Flex User Manual
Working with magnetic particles can be divided into five separate
processes:


collecting magnetic particles



releasing magnetic particles



washing magnetic particles

incubation

 concentration
During the collection of the magnetic particles, the magnetic rod is fully
particles
inside the tip. The magnetic rods together with the tip comb move slowly up
and down in the plate and the magnetic particles are collected onto the
edge of the tips. The magnetic rods together with the tip comb, having
collected the magnetic particles, can be lifted out of the plate and
transferred into the next plate.
After collection of the magnetic particles, the magnetic rods together with
particles
the tip comb are lifted from the plate, the magnetic rods are lifted off and
the tip comb is lowered into the next plate containing a reagent.
Magnetic particles are released by moving the tip comb up and down
several times at considerably high speed until all the particles have been
mixed with the substance in the next reaction.
Washing the magnetic particles is a frequent and an important processing
particles
phase. Washing is a combination of the release and collection processes in
a plate filled with washing solution.
To maximize washing efficiency, the magnetic rods together with the tip
comb are designed to have minimized liquid-carrying properties.
To keep the magnetic particle suspension evenly mixed in long-running
reactions, the tip comb can be moved up and down in the solution.
The volume of the first plate can be larger than the volume of the next
plate, and this is used for concentration purposes (see Figure 2–8 below).
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