Part 4 Working With The Microtome; Preparation And Orientation; Coarse Feed And Trimming Function; Sectioning Instructions - Thermo Scientific HM 450 Instruction Manual

PART 4 WORKING WITH THE MICROTOME

4-1 PREPARATION AND
ORIENTATION
Before sectioning, the specimens must be
prepared appropriately and embedded in suitable
media. Cassettes as well as different forms for
round and rectangular specimens can be used for
embedding.
With the orienting adapter, the specimen can
easily be oriented to the knife.
4-2 COARSE FEED AND
TRIMMING FUNCTION
To adjust approximately the space between
specimen and cutting edge, use the coarse feed
or the trimming function. Continue this to start
trimming the specimen, thus achieving the level of
interest. Any waste should be wiped away in a
forward direction with a brush.
40
Thermo Scientific Sliding Microtome Microm HM 450
4-3
in case of non-compliance with the safety features
of the microtome and when disregarding the
instruction manual.
To cut usable sections, the following points are of
utmost importance:
4-3-1
Only use a sharp knife! If the knife edge is blunt,
move the knife horizontally either to the right or
left side to continue working with the sharp area of
the cutting edge, or have the knife resharpened.
4-3-2
For optimal sectioning, front and back of the knife
must be clean. Especially, paraffin waste must be
removed thoroughly!!
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 760 - English

SECTIONING INSTRUCTIONS

Hazard of hand injuries:
Due to moving parts in connection also
with the microtome knife, a danger area
arises, which might lead to hand injuries
CONDITION OF THE KNIFE
EDGE

CLEAN KNIFE SURFACES