Working With The Microtome; Sectioning Instructions - Thermo Scientific Microm HM355S Operation Manual

Working with the Microtome

Sectioning Instructions

Sectioning
Instructions
Conditions of Knife/Blade
Setting the Cutting Angle
Operation Manual HM335S
60
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Chapter 4
Working with the Microtome
H
AZARD OF HAND INJURIES
Due to moving parts on the knife/blade holder and the extrem sharp-
ness of the microtome knife or blade, working with the microtome
might lead to hand injuries if the safety instructions given in this
manual are not carefully attended to.
Note!
To cut usable sections, the following points are of utmost importance:
ONLY USE A SHARP KNIFE OR BLADE!
• If the cutting edge is blunt, move the knife/blade horizontally either
Edge
to the right or left side to continue working with the sharp area of
the cutting edge, or have the knife re-sharpened or replace the used
blade by a new one.
• For optimal sectioning, front and back of the knife must be clean.
• Especially para n waste must be removed thoroughly!
Angle of the knife [a] in relation to the block surface [b]:
1 Clearance angle a
2 Wedge angle b
3 Upper sharpening
angle g
4 Lower sharpening
angle d
5 Blade angle e
6 Angle of inclination j
[b]
Thermo Fisher Scientific
[a]