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2
Chapter 2 Calibrate the instrument
Verify the instrument performance
Verify the instrument performance
When to perform
the test
About the RNase P
kits
62
When necessary, you can perform the RNase P instrument verification test to confirm
the performance of the QuantStudio
We recommend performing an RNase P instrument verification test:
• After moving the instrument to another location.
• As needed to confirm the function of the instrument.
The RNase P instrument verification test uses instrument verification kits available
from thermofisher.com. The kits differ only in the consumable format for which they
are designed: an RNase P Instrument Verification Plate for QuantStudio
Systems with 96/384-well sample blocks and an Array Card RNase P Kit for the
QuantStudio
™
7 Flex System with array card sample blocks.
RNase P instrument verification plates
The RNase P plate is preloaded with the reagents necessary for the detection and
quantitation of genomic copies of the human RNase P gene (a single-copy gene
encoding the RNase moiety of the RNase P enzyme). Each well contains: PCR master
mix, RNase P primers, FAM
human genomic DNA template.
Figure 5 illustrates the arrangement of the
standard and unknown populations on a
96-well and Fast 96-well RNase P plate.
The RNase P plate contains five replicate
groups of standards (1250, 2500, 5000,
10000, and 20000 copies), two unknown
populations (5,000 and 10,000 copies),
and a no template control (NTC).
Figure 6 illustrates the arrangement of the
standard and unknown populations on a
384-well RNase P plate. The RNase P
plate contains five replicate groups of
standards (1250, 2500, 5000, 10000, and
20000 copies), two unknown populations
(5,000 and 10,000 copies), and a no
template control (NTC).
QuantStudio
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
™
™
6 and 7 Flex Systems.
™
dye-labeled probe, and a known concentration of
Figure 5 96-well and Fast 96-well RNase P
plate layout
Figure 6 384-well RNase P plate layout
™
6 and 7 Flex
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