Appendix C Creating A Custom Pure Dye Plate; Overview - Applied Biosystems 7300 Installation And Maintenance Manual

Overview

The Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System can be used to
run assays designed with custom dyes (dyes not manufactured by Applied Biosystems).
Custom dyes must fluoresce within the spectral range measured by the instrument:
Before using custom dyes with the 7300/7500/7500 Fast instrument, you must:
Materials
Required
Pipettors and pipette tips
(200-µL and 1000-µL)
and Optical 96-Well Fast
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Creating a Custom Pure Dye Plate
• 500 to 650 nm for 7300 systems
• 500 to 700 nm for the 7500/7500 Fast systems
• Determine optimum dye concentration
• Create a custom pure dye plate
• Add the custom dye to the software
• Perform a pure dye calibration (see
Calibration."
Water,
deionized
®
ABI P Optical
RISM
96-Well Reaction Plate
Plate
Chapter 6, "Performing the Pure Dye
Tubes
(2-mL and 10-mL)
Optical Adhesive Cover Powder-free
or Optical Flat Caps
Centrifuge with plate
adapter
Appendix C
Safety
glasses
Custom dye(s)
gloves
GR2170
127
C