Preparing The Reaction Plate - Applied Biosystems 7500 Getting Started Manual

Preparing the Reaction Plate

Standard vs.
IMPORTANT!
Real-Time PCR system. Optical 96-Well Fast Plates will not fit into the standard block
Fast Plates
correctly and will result in loss of data.
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide
Chapter 4 Generating Data from RQ Plates – 7300 or Standard 7500 System
Make sure that you use the standard Optical 96-Well Plate on the 7500
Fast Plates
(PN 4346906)
Notch at
top-left
corner
by A1
30-µL
maximum
reaction
volume
Label the reaction plates, ensuring that you include an endogenous control for each
1.
sample type (for example, each tissue in a study comparing multiple tissues). If
samples are spread across multiple plates, each plate must have an endogenous
control. Additionally, every plate must include an endogenous control for every
sample type on the plate.
Into each well of the reaction plate, add 50 µL of the appropriate PCR master mix.
2.
Keep the reaction plates on ice until you are ready to load them into the 7300/7500
3.
system.
Preparing the Reaction Plate
Standard Plates
d(PN 4306737)
GR2386
7900
HS96 well plate comparison
STANDARD STANDARD STANDARD
Notch at top-right
corner by A12
100-µL
maximum
reaction
volume
4
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