Prepare The Reaction Plate - Applied Biosystems 7500 Getting Started

Prepare the Reaction Plate

Prepare the reactions for each replicate group, then transfer them to the reaction plate
using the plate layout displayed in the 7500 software.
About the For the standard curve example experiment:
Example
Experiment
Notes
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
®
• A MicroAmp Optical 96-Well Reaction Plate is used.
• The reaction volume is 25 µL/well.
• The reaction plate contains:
– 6 Unknown wells
– 15 Standard wells
– 3 Negative control wells
– 72 Empty wells
• The plate layout that the 7500 software automatically generates is used:
Chapter 3 Prepare the Reactions

Prepare the Reaction Plate

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