Preparing Dna; Chapter 3 Setting Up The Reaction Plate - Applied Biosystems 7300 Getting Started Manual

Chapter 3 Setting Up the Reaction Plate

Preparing DNA

Preparing DNA
Systems and
Chemistries for
DNA Isolation
Quality of DNA
Sample Experiment
The meat samples are frozen with liquid nitrogen and ground to a fine powder with a pre-chilled mortar and pestle. DNA is
extracted using the PrepMan
final concentration of 10 ng/µL of DNA for each sample.
Notes
12
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Plus/Minus Assay Getting Started Guide
Applied Biosystems supplies several instrument systems and chemistries for isolating
DNA from a variety of starting materials, such as blood, tissue, cell cultures, plant
material, and food.
™
BloodPrep Chemistry
®
NucPrep Chemistry
™
PrepMan Ultra Sample Preparation Reagent Kit
®
ABI P 6100 Nucleic Acid PrepStation
RISM
For more information, refer to:
• DNA Isolation from Fresh and Frozen Blood, Tissue Culture Cells, and Buccal
Swabs Protocol (PN 4343586)
®
• NucPrep Chemistry Isolation of Genomic DNA from Animal and Plant Tissue
Protocol (PN 4333959)
Ensure that the DNA you use for a plus/minus experiment:
• Has a A ratio of > 1.7.
260/280
• Is extracted from the raw material you are testing using an optimized protocol.
• Does not contain PCR inhibitors.
• Is intact as visualized by gel electrophoresis.
• Has not been heated above 60 ° C, which can cause degradation.
™
Ultra Sample Preparation Reagent Kit (PN 4322547) and protocol (PN 4318925) to obtain a
System Part Number
4346860
4340274
4322547
6100-01