Appendix F Calibration Consumable Preparation; Create A Background Plate Or Array Card; Required Materials - Applied Biosystems QuantStudio 6 Flex User Manual

F
Calibration consumable preparation

Create a background plate or array card

Required
materials
QuantStudio 6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
™
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Create a background plate or array card . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151
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Create a custom dye plate for calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153
Whenever possible, use a Background Plate or the TaqMan
that is included with the spectral calibration kit. The plates/array cards supplied in the
kit contain a buffer that accurately simulates the reagents used for PCR, and,
therefore, produces high-quality calibration data. If a background plate or array card
from a spectral calibration kit is not available, you can create one as described below.
96/384-Well plate sample block
• MicroAmp ™ Optical 96/384-Well Reaction Plate
• Safety glasses
• Optical Adhesive Cover or Optical Flat Caps
• Pipettor, 200-µL (with pipette tips)
• Powder-free gloves
• Deionized water
TaqMan ® Array Card block
• TaqMan ® Array Cards
®
• TaqMan Array Card Sealer
• Centrifuge with array card buckets and array card carrier clips
• Permanent marker or pen
• Pipettor, 200-µL (with pipette tips)
• Powder-free gloves
• Safety glasses
• Deionized water
(QuantStudio 7 Flex System only)
™
®
Array Background Buffer
151