Preparing The Background Calibration Plate - Applied Biosystems 7300 Installation And Maintenance Manual

Chapter 5 Performing the Background Calibration and Optical Calibration

Preparing the Background Calibration Plate

Preparing the Background Calibration Plate
Wear powder-free
IMPORTANT!
gloves when you handle the
background plate.
Retrieve the spectral calibration kit from the
1.
freezer, then remove the prepared background
plate.
Alternatively, create a background calibration
plate as described in
Background Plate."
Return the spectral calibration kit to the freezer.
2.
Allow the background plate to warm to room
3.
temperature (at least 5 minutes).
Remove the background plate from its
4.
packaging.
Do not discard the packaging for
IMPORTANT!
the background plate. The background plate can
be used up to three times if it is stored in its
original packaging sleeve.
Standard plates only: Vortex the plate for
5.
5 seconds. Do not vortex Fast plates.
(Remaining steps apply to both standard and Fast
plates.)
Briefly centrifuge the background plate in a
6.
centrifuge with a plate adapter (<1500xg).
The plate must be well mixed and
IMPORTANT!
centrifuged.
Notes
52
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Appendix B, "Creating a
Ambient >00:05:00
<1500× g 00:00:05
Spectral calibration kit
Background plate