Analyzing And Viewing Rq Plate Data - Applied Biosystems 7500 Getting Started Manual

Analyzing and Viewing RQ Plate Data

To analyze RQ Plate data after the run, click
Starting the
Software 1.3.1 mathematically transforms the raw fluorescence data to establish a
Analysis
comparative relationship between the spectral changes in the passive reference dye and
those of the reporter dyes. Based on that comparison, the software generates four result
views: Plate, Spectra, Component, and Amplification Plot.
In the Results tab, you can view the results of the run and change the parameters. For
About the Results
example, you can omit samples or manually set the baseline and threshold. If you change
Tab
any parameters, you should reanalyze the data.
The Results tab has four secondary tabs, each of which is described below. Details are
provided in the Online Help.
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide
Chapter 4 Generating Data from RQ Plates – 7300 or Standard 7500 System
• To move between views, click a tab.
• To select all 96 wells on a plate, click the upper-left corner of the plate.
• To adjust graph settings, double-click the y- or x-axes of a plot to display the Graph
Settings dialog. The adjustable settings depend on which plot you are viewing.
Analyzing and Viewing RQ Plate Data
or select Analysis > Analyze. The SDS
STANDARD STANDARD STANDARD
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