Preparing The Roi Calibration Plate - Applied Biosystems 7300 Installation And Maintenance Manual

Chapter 4 Performing the Regions of Interest (ROI) Calibration

Preparing the ROI Calibration Plate

Preparing the ROI Calibration Plate
Standard Plates
versus Fast
Plates
Preparing the
Plate
Notes
38
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Use the plate appropriate for your system.
Standard Plates –
7300 and 7500 Systems
Notched corner at top right
A1 at corner
opposite from
notched
top-right
corner (A12).
100-µL
maximum
reaction
volume
Vortex standard plates to ensure complete
mixing, then centrifuge to ensure that all
reagents are contained in the bottom of the
well.
Note:
Optical 96-Well Fast Plates do not fit
correctly into the standard block.
Wear powder-free gloves when you handle the ROI
IMPORTANT!
calibration plate.
In the desktop, double-click the icon for the SDS software (
1.
software.
Fast Plates –
7500 Fast Systems
Notched corner at top left
A1 at
notched
top-left
corner
30-µL
maximum
reaction
volume
Centrifuge Fast plates to ensure that all
reagents are in the bottom of the well.
IMPORTANT!
Do not vortex Fast plates.
Vortexing can cause loss of smaller reaction
volumes.
Note:
Standard plates are not compatible
with the 7500 Fast system and may be
crushed by the 96-Well Fast Block.
) to start the
Double-click