View The Well Table - Applied Biosystems 7500 Getting Started

Real-time pcr system
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View the Well Table

The Well Table displays data for each well in the reaction plate, including:
About the
In the standard curve example experiment, you review the Well Table for:
Example
Experiment
View the Well
Table
2a
Notes
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
• The sample name, target name, task, and dyes
• The calculated threshold cycle (C
values
• Comments
• Flags
• Quantity values
• Flags
• C
values (including C
T
1. In the navigation pane, select Analysis, then select the View Well Table tab.
If no data are displayed, click Analyze.
Note:
2. Use the Group By drop-down list to group wells by a specific category. For the
example experiment, group the wells by replicate, flag, or C
You can select only one category at a time.
Note:
In the Group By drop-down list, select Replicate. The software groups the
a.
replicate wells: negative controls, standards, and samples. In the example
experiment, note that the quantity values within each replicate group are similar.
Note:
To move a column, drag the column heading.
Chapter 5 Analyze the Experiment
), normalized fluorescence (Rn), and quantity
T
standard deviation)
T

View the Well Table

value.
T
81

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