Omit Wells From The Analysis - Applied Biosystems 7500 Getting Started

Chapter 5 Analyze the Experiment

Omit Wells from the Analysis

Omit Wells from the Analysis

About the
Example
Experiment
Omit Wells
4a
5
Notes
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Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experimental error may cause some wells to be amplified insufficiently or not at all.
These wells typically produce C
associated replicate wells. If included in the calculations, these outliers can result in
erroneous measurements. To ensure precision, omit the outliers from the analysis.
In the standard curve example experiment, well B12 is flagged as a potential outlier.
1. In the navigation pane, select Analysis
If no data are displayed, click Analyze.
Note:
2. In the Amplification Plot screen, select C
3. Select the View Well Table tab.
4. In the Well Table:
In the Group By drop-down list, select Replicate.
a.
Look for any outliers in the replicate group (be sure they are flagged). In the
b.
example experiment, the 7500 software flagged well B12 as a potential outlier.
3
5. Select the Omit check box next to well B12.
values that differ significantly from the average for the
T
Amplification Plot.
vs Well in the Plot Type drop-down list.
T
Experiments