Omitting Samples From A Study - Applied Biosystems 7500 Getting Started Manual

Omitting Samples from a Study

Experimental error may cause some wells to amplify
insufficiently or not at all. These wells typically
produce C values that differ significantly from the
T
average for the associated replicate wells. If included
in the calculations, these outlying wells (outliers) can
result in erroneous measurements.
To ensure precise relative quantification, you must
carefully view replicate groups for outliers. You can
remove outliers manually using the C
Position Amplification Plot.
To remove samples from an RQ Study:
Select the Amplification Plot tab.
1.
In the Data drop-down list, select Ct vs. Well
2.
Position.
In the RQ Detector grid, select a detector to
3.
examine. All samples that use this detector are
displayed in the RQ Samples grid.
In the RQ Samples grid, click to select the
4.
samples to display in the Amplification Plot.
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide
vs. Well
T
Chapter 6 Analyzing Data in an RQ Study
Omitting Samples from a Study
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