Ez Prep Operation; Column Mounting - Teledyne CombiFlash Rf User Manual

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CombiFlash® Rf User Manual

5.5 EZ Prep Operation

Flash separations on the EZ Prep system are identical to those
performed on the Rf
same control capabilities as the flash separations with a few
minor differences.

5.5.1 Column Mounting

Note
HPLC columns that have less than 100 psi back pressure may not
function reliably on the EZ Prep. This may occur with columns
using 20 µm packing and low viscosity solvents such as hexane.
The HPLC column is mounted on the right side of the EZ Prep
system. Use of a guard column is recommended to protect and
extend the life of the HPLC column.
To install a guard column:
1. Remove the union installed on the preparative column
tubing on the right side of the instrument.
2. .Take the tubing supplied with the guard column and
connect it to the guard column outlet.
3. Connect the line that exits from the prep inject valve to the
guard column inlet.
4. Remove the cap on top of the column mounting tower by
grasping the tabs on the front and back of the cover.
5. Place the guard column in the top of the tower, orienting it
so that the inlet is towards the rear of the instrument. The
inlet and outlet tubing for the guard column can be placed
into slots provided in the tower.
6. Replace the cap on top of the tower.
7. Proceed with installing the preparative column.
To install a HPLC Column:
1. If not using a guard column, remove the union from the
tubing.
2. Lift the upper column mount up to allow the column to fit
in the mount.
5-16
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. Preparative HPLC separations have the