Teledyne CombiFlash Rf User Manual page 143

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CombiFlash® Rf User Manual
6.3.2.6 Peak Detection
This section of the window contains option buttons to enable
and disable various peak detection options. When an option is
enabled, the window also allows you to modify the setting
details for that peak detection option. All enabled options will
be displayed on the chromatogram. The options and settings
for the Rf+ systems, including those equipped with 4x, are
described below.
Note
Note: You can select up to four peak detection options on the Rf+
systems, including those equipped with 4x. If using more than one
option, such as 1 with  2, the system considers a peak to be
present when any option is true (a logical OR).
• 1 and 2 (wavelength 1 and 2) — Enable these
options to use and configure primary and secondary
wavelength detection. 2 is set to monitor by default.
When enabled, type or select the peak detection
wavelength in nanometers, then click the Details button
to configure additional settings described below.
• Slope Based — Select this option to enable
Slope-based peak detection. When enabled, peaks will
be recorded if the slope algorithm indicates a peak
within the Peak Width setting.
• Peak Width — Select the average peak width
setting. Peak widths are measured at the baseline.
The slope detector will detect peak widths ranging
from about 0.2 to 2 times the peak width setting. For
example, if you entered a peak width of 1 minute, the
range would be 12 seconds to 2 minutes. For best
operation, the peak width should be set to just over
the average peak width being separated. For
instance, if the average peak width is 45 seconds, you
should enter a peak width of 1 minute. For most flash
chromatography, 1 minute is a good starting point for
a peak width.
• Threshold Detection — Select this option to enable
Threshold peak detection. When enabled, peaks will
be recorded if the Absorbance Units (AU) value is
exceeded.
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