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Section 2 Preparation: Rf + , Rf + Lumen, Rf+ PurIon, and EZ Prep Systems
Note
The system is shipped with common solvent names already
loaded. If your solvent names are listed in the solvent drop-down
list boxes, skip steps 1 through 3.
1. Click the "Add Solvent" button.
2. Enter the solvent name and click the OK button.
3. Repeat steps 1 and 2 for all solvents.
4. For the Solvent 1 drop-down list box, select the solvent
name connected to the 1 Solvent Inlet Port. Repeat for
Solvents 2, 3, and 4.
5. Select the "Enable solvent level sensing" check box to
enable this feature (recommended). Clear this box to
disable this feature.
The solvent level sensing feature will monitor solvent
usage to minimize the risk of running out of solvent during
a purification run. The system compares the volume
required for a purification run with the estimated volume
in the solvent container and alerts you when there is not
enough.
6. If using the solvent level sensing feature, enter the
Minimum Solvent Level (density dependent) as a range in
centimeters.
To understand this range, consider that dichloromethane is
more dense than hexane. If the selected range is 2–4, the
system triggers an alert when the dichloromethane level
falls to about 2 cm above the weighted filter. At the same
selected range, the system would trigger an alert when the
hexane falls to about 4 cm above the weighted filter.
2.13.2 Enable Automatic Phase Change (EZ Prep Only)
The EZ Prep has a feature which automatically changes
solvents used in normal phase flash separations to HPLC
reverse phase separations and vice versa without any user
interaction. For this feature to function properly, the strong
reverse phase solvent must be miscible with the strong normal
phase solvent. In addition, specific solvent types and locations
must be followed. To enable this feature, simply press the
button. A dialog box will appear that defines placement of the
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