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CombiFlash® Rf
User Manual
Use ELSD by Default - causes all runs to use the ELSD
detector unless disabled in the run requirement screen
[S
TARTING A RUN USING DEFAULT SETTINGS [6.4.1]
2.13.8 Prep HPLC Tab
The
DEFAULT COLUMN
used Preparative HPLC column. This will be the method
loaded when the user first selects use of the Preparative HPLC
function during normal operation. Many times this will be the
column mounted onto the system. The drop down list to select
the
DEFAULT COLUMN
by the user. To define a column, refer to the area below the
DEFAULT COLUMN
To define a column:
1. Press N
column to be named. This name will be used to refer to the
column in normal usage. This name will also be printed on
the report.
2. Select the
chromatograms for future reference.
3. Enter a
by the system to recommend an optimal flow rate to
achieve highest resolution for the column being defined.
4. Enter a
separation length. This length is based on a gradient
segment approximately 12 column volumes in duration.
5. Select
defined by the system. This is an opportunity to edit the
recommended gradient shape and duration to better match
the expected chemistry. A higher flow rate can be selected
to balance resolution and throughput.
6. Selecting S
on the system.
7. If you are done defining columns, press the
If you have several columns that will be used on the system,
these can be defined at this time or at any later time.
• S
AVE
based on an existing column. This is done by selecting
the existing column in the C
2-24
allows selection of the most commonly
is based on the columns already defined
field.
. This will open a dialog box allowing the new
EW
MEDIA SIZE
COLUMN DIAMETER
COLUMN LENGTH
DEFINE METHOD
. This will save the column and allow its use
AVE
A
allows you create a new column definition
S
. This data will be printed on the
. This information will be used
. This is used to recommend a
. This allows a review of the method
N
OLUMN
].
button.
OK
drop down
AME
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