Teledyne CombiFlash Rf User Manual page 133

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CombiFlash® Rf User Manual
Main window dynamically changes to display the controls
required for the current state of the system.
The top region of the Main window always displays a
G
RADIENT
displays the R
, and P
[6.3.1.3]
the left side of the window changes to show the collection rack
map or the method parameters in a P
pane.
[6.3.1.5]
Note
Note: The main window is expanded for CombiFlash Rf 4x
systems. The main window the displays the settings and views in
four tabs, one for each column channel. A fifth tab — B
— contains the controls to run sequential separations.
6.3.1.1 Gradient Plot
The gradient plot area depicts the current gradient that will
be used for the run. The X-axis of the plot displays the run
time in minutes or C
can be adjusted by typing or selecting the Run Length. The
left Y-axis displays absorbance units and the right Y-axis
indicates the percentage of solvent B. By default, PeakTrak
automatically scales the left Y-axis to best display the
absorbance trace. PeakTrak also sets the right Y-axis to 100%.
You can override the Y-axes scales by clicking the Format
Graph button and setting the desired values.
The Cartridge Bypass button may be used during a run to
relieve back pressure caused by impurities precipitating in the
solid load cartridge. Use this feature with care to avoid leaving
desired compound on the cartridge. Read more about this
feature in B
If the external detector option is enabled on Rf+ systems, the
plot area will include a secondary y-axis to display the analog
voltage trace. If the evaporative light scattering detector
(ELSD) is enabled on an Rf+ Lumen system, a secondary left
Y-axis is also included. PurIon systems will display a
secondary y-axis which displays ion count for each single ion
or range chosen.
The gradient plot area also provides a convenient method to
edit the gradient curve. You can click and drag any of the
6-12
P area. The bottom region of the window
LOT [6.3.1.1]
S
UN ETTINGS [6.3.1.2]
C
EAK OLLECTION
OLUMN
YPASSING THE
, R C
UN
B
UTTONS [6.3.1.4]
EAK
V
OLUMES [
-
ON LINE
S L C
OLID OAD ARTRIDGE [6.4.7]
B
ONTROL UTTONS
. After a run,
C D
OLLECTION
ATCH [6.3.5]
. The X-axis scale
]
ATA