Manual Control Of The Combiflash Rf - Teledyne CombiFlash Rf User Manual

wavelength. See the M
[6.4.9]
• Green traces are from ELSD (Lumen systems) or
external detectors.
• The PurIon traces are color coded with the selected
masses below the graph y-axis.
• The blue gradient curve that was used during the run.
The % Solvent B scale is shown in blue on the right
Y-axis.
• The X-axis depicts the run time, shown as minutes or
C
OLUMN
• Vertical lines appear at intervals along the X-axis. These
lines indicate collection tube changes. To prevent the
plot area from being obscured by tube change marks,
PeakTrak may limit the number of marks.

6.4.11 Manual Control of the CombiFlash Rf

Manual Control can assist with method development,
maintenance of the system plumbing, and system
troubleshooting. To manually control the CombiFlash Rf
system, first open the M
clicking the Tools>Manual Control menu command.
6.4.11.1 Pumping Solvents
To pump either solvent, select the solvent from the Solvent A
or Solvent B lists. Then, click the Prime A or Prime B buttons
to pump the selected solvent. To pump a mixture or Solvent A
and B, adjust the Percentage Solvent B setting and then click
the Prime __% B button. When finished, click the Stop button.
You can control the flow rate by adjusting the Flow Rate
mL/min setting.
By default, the system pumps the solvent directly to the waste
port. With Rf+ systems, including those equipped with 4x, you
can change the fluid path by selecting a different Valve
Position option (through column or through cartridge and
column). For valve positions other than Direct To Waste, you
can pump the solvent into a collection tube. To do so, select the
Pump Into Tube # option and then select which tube ("next" or
specific tube number).
help topic.
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Section 6 User Help Reference
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ANUAL ONTROL [6.3.12]
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