Table of Contents
Advertisement
+
CombiFlash® Rf
User Manual
estimate during operation. Solvent level sensing is not available on
the Rf 150 system.
Note
If the waste level sensing tube is inserted correctly into the waste
container, Rf
automatically suspend operation before an overflow condition
might exist. To prevent the run from being suspended before
completing the run, ensure that the container will hold the
expected waste volume. Waste level sensing is not available on
the Rf 150 system.
+
7. (Rf
that the ELSD option is selected. Otherwise the ELSD may
be disabled. The Rf
check this option if the previous run used the ELSD.
Note
If you typically use normal phase solvents and the ELSD P-trap
drain fluid hasn't been replenished recently, you may need to
ensure the P-trap is full for maximum ELSD signal strength. This
can be done by lifting the drain line above the instrument and
placing 10 ml of isopropyl alcohol in the drain line. Lift the line
sufficiently that the fluid flows into the P-trap drain. Then place into
a waste container so that the excess fluid can drain out of the trap.
If you normally use higher boiling point solvents such as water, the
P-trap will typically remain full and will not require replenishment.
Note
If you are using a silica flash column and your solvent exceeds 7.5
pH, the ELSD should not be used. Basic conditions can dissolve
silica. When the solvent with dissolved silica is evaporated in the
ELSD, silica can build up over a period of time and eventually clog
the ESLD, resulting in an expensive repair. Sample build up in the
spray chamber is less of a concern since it is sent to the chamber
in smaller quantities only during the peak and is more easily
cleaned from the chamber during regular cleaning (see
Chromatography Technical Note TN21, Preventative cleaning of
the ELSD detector).
5-8
+
systems, including those equipped with 4x, will
Lumen) If the ELSD will be used for the run, ensure
+
Lumen system will automatically
Advertisement
Table of Contents
