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CombiFlash® Rf
User Manual
2.14 Prime the Solvent Lines
Before the first use, the system should be primed. Ensure that
the solvent containers are filled, then:
1. Select the "Tools>Auto prime" menu command.
2. There are 4 solvent selection lists labeled from 1st solvent
through 4th solvent, describing the order in which solvents
will be pumped. The solvent choices for each list are those
defined by the CONFIGURATION [6.3.10] window. (Rf
systems, including those equipped with 4x).
• If all solvents are of a similar polarity, it is
recommended that the solvent be primed in order of
decreasing solvent strength, with the weakest solvent
primed last.
• If the 4 solvents are of mixed polarity (aqueous and
nonpolar), ensure that sequential solvents are
compatible, such as water, acetonitrile, ethyl acetate,
hexane.
• If you are performing an autoprime after redefining
some of the solvents, it may be beneficial to run 2
autoprime operations sequentially to get full flushing
of the lines.
3. Click the P
Preparative HPLC systems typically require the user to open
a prime valve and draw fluid with a syringe to prime the
system. The EZ Prep doesn't require this action due to its
unique valves and integrated priming pump.
Note
When priming the first time, inspect the solvent and waste
connections to the system. If any leaks are observed, click the
Cancel button to stop the Auto Prime. Correct the leak by
tightening the fitting an additional
Prime from step 1.
To terminate an autoprime step in process and advance to
the next Auto Prime step, you can click the Fast Forward
button. The Auto Prime window closes when finished.
After Auto Priming, the system is ready for operation. If you
are priming a new system for the first time, it may be
2-26
button to start priming the system.
LAY
1
turn, and then restart the Auto
4
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