Teledyne CombiFlash Rf User Manual page 181

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CombiFlash® Rf
User Manual
detectors except the mass spectrometer. The
threshold levels are color-coded to the detector trace.
• MS - Click this button to display a mass spectrum
(PurIon systems only). Clicking in the Chromatogram
window displays a mass spectrum at that point
during the run. Clicking on a tube in the rack map
shows the mass spectrum of the contents of that
fraction.
• UV (will be displayed as vis on UV-vis equipped
systems) - Click this button to display a UV or
UV-visible spectrum (UV-vis systems only). Clicking
in the Chromatogram window displays a mass
spectrum at that point during the run.
• Extract Method button — Click the Extract button to
load a new method file based on the parameters for the
run you are viewing.
• Close button — Click the Close button when you are
done viewing the run.
6.4.10.1 General instructions for reading the data
Most elements on the window are color-keyed to help you
locate the tubes containing the peaks of interest. Below each
collected peak there is a color bar that matches a tube in the
map on the left. The tube map provides a visual
representation of the tubes that contain the peaks of interest.
If you wish to identify the tube by number, refer to the table
below the rack diagram.
If the run used multiple racks, the Previous and Next Rack
buttons are active. Click these buttons to scroll through the
available racks. The number of the currently displayed rack is
shown at the top left of the window.
6.4.10.2 Reading the Chromatogram
The plot area displays the following:
• The red absorbance trace produced by the system's peak
detector. Absorbance units (also shown in red) that
correspond to this trace are shown on the left Y-axis.
• Purple absorbance and green purity measurement
traces may be visible if you are monitoring a second
6-60

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