Teledyne CombiFlash Companion XL Installation Manual
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Teledyne CombiFlash Companion XL Installation Manual

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CombiFlash
Companion
XL
®
®
Installation Guide
Indoor Use Only
COPYRIGHT © 2002 by
Teledyne Isco, Inc.
4700 Superior Street
Lincoln, Nebraska, U.S.A. 68504
Phone: (402) 464-0231
Toll Free: (800) 228-4373
FAX: (402) 465-3022
Released: January 5, 2005
Revision H, April 28, 2009
Part #69-5243-040
i

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  • Page 1 CombiFlash Companion ® ® Installation Guide Indoor Use Only COPYRIGHT © 2002 by Teledyne Isco, Inc. 4700 Superior Street Lincoln, Nebraska, U.S.A. 68504 Phone: (402) 464-0231 Toll Free: (800) 228-4373 FAX: (402) 465-3022 Released: January 5, 2005 Revision H, April 28, 2009...
  • Page 2 CombiFlash® Companion® XL Installation Guide...
  • Page 3 Installation Flowchart Read Chapter 1 for safety and general instructions. Install the Companion XL. See Chapter 2 Optional touch-panel display Choose a connection type: Direct Connection Network to a Computer Connection via Ethernet Port Windows 98 See Chapter 7 Computer See Chapter 3 Windows NT Computer...
  • Page 4 CombiFlash® Companion® XL Installation Guide...

  • Page 5: Table Of Contents

    ® ® CombiFlash Companion Installation Guide Table of Contents 1. Introduction 1.1 Documentation Overview ..... . 1-1 1.2 Product Overview ......1-2 1.3 Operating Overview .
  • Page 6 ® ® CombiFlash Companion XL Installation Guide 4. Direct Connection with Windows NT4 4.1 Windows NT4 Computer Requirements ..4-1 4.2 Establishing a Direct Connection ....4-1 4.2.1 Configure the Computer’s Local Area Network .
  • Page 7 Table of Contents Operation and Maintenance 8. Operation 8.1 Installing a Collection Rack ....8-1 8.2 Prime the Solvent Lines ..... . . 8-2 8.3 Loading a RediSep Column .
  • Page 8 ® ® CombiFlash Companion XL Installation Guide viii...

  • Page 9: Introduction

    ® ® CombiFlash Companion Installation Guide Section 1 Introduction 1.1 Documentation Overview This installation guide provides: • Safety information • Unpacking instructions • Installation instructions, including — • Direct connection to a computer • Placing the Companion XL on a network The installation procedure will vary according to the intended instrument control method.

  • Page 10: Product Overview

    Companion XL at the instrument, from a computer in the lab, or from remote locations. ® The Companion XL uses Teledyne Isco RediSep columns. Compatible sizes range from 120 to 1500 grams, which can accept sample sizes from 0.1 to 150 grams.
  • Page 11 Section 1 Introduction Figure 1-1 CombiFlash Companion XL with Foxy 200 Fraction Collector (shown with optional touch-panel display)

  • Page 12: Operating Overview

    Companion XL through a network using TCP/IP protocol. Note Teledyne Isco recommends that you obtain assistance from your IT department before attempting direct or network connections to a Windows computer. 1.3.1 Control Priorities The Companion XL supports multiple connections.

  • Page 13: Specifications

    Section 1 Introduction 1.4 Specifications Table 1-1 Specifications Dimensions Height: 64 cm (25") Width: 37 cm (14.5") Depth: 40 cm (15.75") Weight 23.5 kg (51.7 lbs) Input voltage range from 100 to 240 VAC, 2.0 Power Options Amps maximum Line Frequency 50/60 Hz Ambient Temperature 20 to 40°C (maximum temperature must be...

  • Page 14: Controls, Indicators, And Features

    Figures 1-2 and 1-3 illustrate the controls on the CombiFlash ® Companion XL. Refer to the Foxy 200 instruction manual for complete information on the fraction collector. This manual is available in PDF format on the documentation CD. Figure 1-2 CombiFlash Companion XL (Front)
  • Page 15 Section 1 Introduction 1. Status LEDs – The POWER LED lights when power is applied to the system. The READY LED illuminates after the Companion XL completes its startup routine and is ready for operation. 2. Disconnect Device (Power Switch) – Located on side of unit.
  • Page 16 ® CombiFlash Companion XL Installation Guide Figure 1-3 CombiFlash Companion XL (Back) 10. Top Shelf Drain – Spills on the top shelf are drained through this tubing. 11. Foxy 200 Control Port – Serial port for connection to Foxy 200 Fraction Collector.

  • Page 17: Safety

    Section 1 Introduction 14. Foxy 200 Fluid Port – Routes fluid output to the fraction collector. 15. Cooling Fan – Ventilates and cools the Companion XL. 16. Rear Panel Knob – Service personnel only. Used to open the hinged rear panel to access internal components. 17.

  • Page 18: Hazard Severity Levels

    OSHA Guide 1910.1000. To reduce those levels to a safe exposure, Teledyne Isco recommends that you place the system in a laboratory hood designed for the purpose of ventilation. This hood should be constructed and operated in accordance with federal, state, and local regulations.

  • Page 19: Hazard Symbols

    Section 1 Introduction 1.6.2 Hazard Symbols The equipment and this manual use symbols used to warn of hazards. The symbols are explained in Table 1-2. Table 1-2 Hazard Symbols Warnings and Cautions The exclamation point within the triangle is a warning sign alerting you of important instructions in the instrument’s technical reference manual.

  • Page 20: For Additional Information

    ® ® CombiFlash Companion XL Installation Guide 1.7 For Additional Information Technical assistance for the CombiFlash Companion XL can be obtained from: Teledyne Isco, Inc. 4700 Superior St. Lincoln NE 68504 Phone: (800) 228-4373 or (402) 464-0231 Fax: (402) 465-3001 E-mail: Iscoservice@Teledyne.com...

  • Page 21: Installation

    WARNING If there is any evidence that the system has been damaged in shipping, do not plug it into the power line. Contact Teledyne Isco or its authorized representative for advice. Compare the contents of the boxes with the enclosed packing slip.

  • Page 22: Instrument Location

    Companion XL Installation Guide 2.3 Instrument Location The CombiFlash Companion XL has a footprint of about 1500 ) of bench space (37 cm W × 40 cm square centimeters (230 in D, 14.5" W × 15.75" D). Ensure that the Companion XL has at least 3 cm (1.25") of air space behind it for ventilation.

  • Page 23: Electrical Connections

    Section 2 Installation 2.4 Electrical Connections 2.4.1 Foxy 200 Control Cable Locate the serial cable, P/N 480-7996-00, in the accessory kit. Attach this cable’s 9-pin connector to the Foxy 200 Control Port on the rear panel of the Companion XL. Attach the other end to the 25-pin connector labeled “SERIAL”...

  • Page 24: Connect Drain Tubing

    ® ® CombiFlash Companion XL Installation Guide Attach tubing to the Foxy 200 port on the back of the Companion XL and route it to the inlet port on the Foxy 200’s Diverter Valve. Attach tubing to the Diverter Valve’s Waste port and route it to a suitable collection container.
  • Page 25 Section 2 Installation The other drain is located on the top shelf of the Companion XL. Route the tubing attached to the bottom of this drain (Figure 2-2) to a suitable waste fluid collection container. This will carry away spills on the top shelf. Note It may be necessary to extend the length of tubing from both locations.
  • Page 26 ® ® CombiFlash Companion XL Installation Guide...

  • Page 27: Direct Connection With Windows 98

    ® ® CombiFlash Companion Installation Guide Section 3 Direct Connection with Windows 98 This section provides instructions for establishing direct communications with the Companion XL from a computer with Windows 98 operating system. 3.1 Windows 98 Computer Requirements Minimum System Requirements: •...
  • Page 28 ® ® CombiFlash Companion XL Installation Guide 3. Highlight the “TCP/IP” item in the list, and click on the “Properties” button. The TCP/IP Properties Window will open (Figure 3-2). Select the IP Address tab. Note The following steps change the local area connection settings. If the computer must be restored to its original network configuration, record the present Internet Protocol (TCP/IP) settings now.

  • Page 29: Options

    Section 3 Direct Connection with Windows 98 Figure 3-2 TCP/IP Properties Window 3.2.2 Configure the Computer’s Internet Browser Options 1. Start Internet Explorer. 2. From the File menu, select “Work Offline.” 3. From the Internet Explorer window, select the “Tools>Internet Options...” menu command. 4.
  • Page 30 ® ® CombiFlash Companion XL Installation Guide 10. On the Internet Options window, click Apply to change the settings. 11. On the Internet Options window, select the Connections tab. a. Click the LAN Settings... button. b. Ensure that the “Use a Proxy Server” check box is not selected.

  • Page 31: Start Direct Communications

    Section 3 Direct Connection with Windows 98 Note The “Use a Proxy Server” check box must be cleared to connect to the Companion XL. c. Click OK to close the LAN settings window. 12. Click OK to close the window. 13.
  • Page 32 ® ® CombiFlash Companion XL Installation Guide Note Some versions of the Windows operating system will require the computer to be restarted before new ActiveX plug-ins can run. If you are notified that the computer must be restarted, you may do so now.

  • Page 33: Direct Connection With Windows Nt4

    ® ® CombiFlash Companion Installation Guide Section 4 Direct Connection with Windows NT4 This section provides instructions for establishing communications with the Companion XL from a computer with Windows NT4 operating system. 4.1 Windows NT4 Computer Requirements Minimum System Requirements: •...

  • Page 34: Configure The Computer's Local Area

    ® ® CombiFlash Companion XL Installation Guide 4.2.1 Configure the Computer’s Local Area Network 1. From the Windows Start button, select “Settings>Control Panel” to view the control panels. 2. Locate the “Network” icon and open this control panel. 3. View the Protocols tab of the Network window and highlight the “TCP/IP Protocol”...

  • Page 35: Options

    Section 4 Direct Connection with Windows NT4 Figure 4-2 TCP/IP Properties 4.2.2 Configure the Computer’s Internet Browser Options 1. Start Internet Explorer. 2. From the File menu, select “Work Offline.” 3. From the Internet Explorer window, select the “Tools>Internet Options...” menu command. 4.
  • Page 36 ® ® CombiFlash Companion XL Installation Guide 10. On the Internet Options window, click Apply to change the settings. 11. On the Internet Options window, select the Connections tab. a. Click the LAN Settings... button. b. Ensure that the “Use a Proxy Server” check box is not selected.

  • Page 37: Start Direct Communications

    Section 4 Direct Connection with Windows NT4 Note The “Use a Proxy Server” check box must be cleared to connect to the Companion XL. c. Click OK to close the LAN settings window. 12. Click OK to close the window. 13.
  • Page 38 ® ® CombiFlash Companion XL Installation Guide 6. The Companion XL begins to download the necessary files for operation. 7. When the download is complete, the Companion XL will display the PeakTrak Main window. The title bar will read “Companion Powered by PeakTrak.” Note Some versions of Windows operating system will require the computer to be restarted before new ActiveX plug-ins can run.

  • Page 39: Direct Connection With Windows 2000

    ® ® CombiFlash Companion Installation Guide Section 5 Direct Connection with Windows 2000 This section provides instructions for establishing communications with the Companion XL from a computer with Windows 2000 operating system. 5.1 Windows 2000 Computer Requirements Minimum System Requirements: •...
  • Page 40 ® ® CombiFlash Companion XL Installation Guide 5.2.1 Configure the Computer’s Local Area Network 1. From the Windows Start button, select “Settings>Control Panel” to view the control panels. 2. Locate the “Network and Dial-up Connections” icon and open this control panel. 3.

  • Page 41: Options

    Section 5 Direct Connection with Windows 2000 Figure 5-2 Internet Protocol Properties Window 7. Enter the default Subnet Mask “255.255.255.0.” 8. Click the OK button to close the Internet Protocol properties window. Then, click the OK button to close the Local Area Connection properties window.
  • Page 42 ® ® CombiFlash Companion XL Installation Guide Figure 5-3 Default Internet Security Options 6. Click the “Custom Level” button. The Security Settings options will appear. 7. In the Reset Custom Settings box, select “Medium” from the pull-down list and click the Reset button. 8.

  • Page 43: Start Direct Communications

    Section 5 Direct Connection with Windows 2000 Figure 5-4 ActiveX Security Settings Note The “Use a Proxy Server” check box must be cleared to connect to the Companion XL. c. Click OK to close the LAN settings window. 12. Click OK to close the window. 13.
  • Page 44 ® ® CombiFlash Companion XL Installation Guide 4. Start Internet Explorer and type in the address “http://192.168.1.51/login”. The browser will display the PeakTrak Login screen. Note The PeakTrak Login screen indicates that the direct connection is working. 5. Enter the login password for the Companion XL. The Companion XL is shipped with the default password of “companion”...

  • Page 45: Direct Connection With Windows Xp

    Installation Guide Section 6 Direct Connection with Windows XP This section provides instructions for establishing direct communications with the CombiFlash Companion XL from a computer with Windows XP operating system. 6.1 Windows XP Computer Requirements Minimum System Requirements: • 266 MHz Pentium processor •...
  • Page 46 ® ® CombiFlash Companion XL Installation Guide 6.2.1 Configure the Computer’s Local Area Network 1. From the Windows Start button, open the Control Panel. 2. Locate the Network Connection icon and open this control panel. 3. Highlight the “LAN or High-speed Internet Connection” icon.

  • Page 47: Options

    Section 6 Direct Connection with Windows XP Figure 6-3 Alternate Configuration Settings 4. Highlight the “Internet Protocol (TCP/IP)” item in the list, and click on the “Properties” button. Refer to Figure 6-2. 5. Select the Alternate Configuration tab. Click the “User Configured”...
  • Page 48 ® ® CombiFlash Companion XL Installation Guide Figure 6-4 Default Internet Security Settings 3. From the Internet Explorer window, select the “Tools>Internet Options...” menu command. 4. Select the Security tab on the Internet Options window. 5. Highlight the Internet icon. 6.

  • Page 49: Start Direct Communications

    Section 6 Direct Connection with Windows XP Figure 6-5 ActiveX Security Settings 11. On the Internet Options window, select the Connections tab. a. Click the LAN Settings... button. b. Ensure that the “Use a Proxy Server” check box is not selected.
  • Page 50 ® ® CombiFlash Companion XL Installation Guide Note Logging in with administrator privileges is necessary to register PeakTrak software components (during step 6) on the computer so that all user accounts on that computer will be able to connect to the Companion XL. 4.

  • Page 51: Networking

    ® ® CombiFlash Companion Installation Guide Section 7 Networking This section provides instructions for adding the CombiFlash Companion XL to a local area network and connecting to it from a Windows computer. Note The procedures described in this section will require a user account on the PC with Administrator privileges.
  • Page 52 ® ® CombiFlash Companion XL Installation Guide area network. The IP address is assigned by your network administrator. • Enter Netmask – Use this to change the Companion XL’s Netmask setting. The Netmask is assigned by your network administrator. • Enter Gateway Address – Use this to set a gateway for the Companion XL.

  • Page 53: Install Network Cable

    Section 7 Networking 7.2.2 Install Network Cable 1. Turn the Companion XL’s power switch to OFF. 2. Disconnect the blue crossover cable from the Companion XL. (This cable may only be used for direct connections.) 3. Connect the Companion XL to the local area network using the beige Ethernet cable (P/N 480-6545-01).
  • Page 54 ® ® CombiFlash Companion XL Installation Guide Table 7-1 Minimum Computer Requirements Compatible Windows 98 Operating Systems Windows NT4 Windows 2000 with Service Pack 3 Windows XP Professional Processor Pentium class, 266 MHz minimum Memory Must meet Microsoft’s minimum recommended RAM for the selected operating system.
  • Page 55 Section 7 Networking Figure 7-1 Default Internet Security Options Figure 7-2 ActiveX Security Settings...

  • Page 56: Area Network

    ® ® CombiFlash Companion XL Installation Guide 12. In Internet Explorer, type in the Companion XL’s address assigned by your network administrator “http://______________/login”. The browser will display the PeakTrak login screen. Note The PeakTrak Login screen indicates that the Companion XL has been successfully added to the local area network.

  • Page 57: Operation

    ® ® CombiFlash Companion Installation Guide Section 8 Operation This section provides abbreviated operating instructions for the Companion XL. For complete instructions, refer to PeakTrak’s on-line help. 8.1 Installing a Collection Rack Before beginning a run, you must load collection tubes on the Foxy 200.

  • Page 58: Prime The Solvent Lines

    ® ® CombiFlash Companion XL Installation Guide 3. Place the racks on the Foxy 200 (Figure 8-1). 4. If this is the first time the system has been used, or if a different rack has been selected, verify the configuration setting.

  • Page 59: Loading A Redisep Column

    Section 8 Operation Note A priming tube is a piece of tubing with Luer connectors that attach between the column mounts, bypassing the column. The Companion XL is shipped with a priming tube installed. 4. Click the “Ok” button to start priming the system. The Companion XL primes pump B, then pump A.
  • Page 60 Use the RediSep 120 g column. RediSep 120g Normal Phase columns can be ordered in packages of 10. (Teledyne Isco Part Number 68-2203-024) 0.33 – 33g Samples Use the RediSep 330 g column. RediSep 330g Normal Phase columns can be ordered in packages of 4.
  • Page 61 Section 8 Operation Figure 8-3 Loading a 330g RediSep Column Note PeakTrak will automatically detect the column size. The detected column size is displayed on the PeakTrak Main and PeakTrak Advanced Program windows.

  • Page 62: Prepare The Sample

    ® ® CombiFlash Companion XL Installation Guide 8.4 Prepare the Sample Before starting the run, the sample should be prepared for introduction to the column. The Companion XL supports three methods that introduce the sample: liquid sample injection, solid sample cartridges, and loading sample directly onto the column.
  • Page 63 Section 8 Operation Figure 8-5 Sample Type set for Liquid on PeakTrak’s Main window 2. When all settings have been configured, click the Play button to start the run. After column equilibration, the system pauses for the liquid injection (Figure 8-6). 3.
  • Page 64 ® ® CombiFlash Companion XL Installation Guide Figure 8-6 Inject Sample display Note Chase solvent clears the Liquid Injection accessory tubing and forces the liquid compound mixture onto the column. Without the chase solvent, some of the sample will remain in the tubing and will not be separated.
  • Page 65 Section 8 Operation See Inset (below) Figure 8-7 Syringe connected to the Liquid Injection accessory...

  • Page 66: Solid Sample Cartridges

    ® ® CombiFlash Companion XL Installation Guide 8.4.2 Solid Sample Cartridges Solid sample cartridges are practical when there is a need to introduce a low-solubility sample onto the column. The cartridge holds the prepared sample. After equilibration, the Companion XL changes the fluid path so that the mobile phase passes through the cartridge and onto the column.
  • Page 67 Section 8 Operation Figure 8-9 Fitting the cap on the cartridge Figure 8-10 Installing the solid sample cartridge 8-11...

  • Page 68: Direct Loading A Sample

    ® ® CombiFlash Companion XL Installation Guide 8.4.3 Direct Loading a Sample Direct loading places the sample mixture on the column media. If using this technique, remove the column inserted in section 8.3 of these instructions. Then, transfer the mixture onto the column through the inlet fitting and re-insert the column.

  • Page 69: Operation During The Purification Run

    Section 8 Operation • Liquid—Select this option when you will be injecting a liquid sample into the injection port. • Solid—Select this option when you will be using a solid sample cartridge. • Direct —This option is useful when a sample is direct loaded on the column media.
  • Page 70 ® ® CombiFlash Companion XL Installation Guide 8-14...

  • Page 71: Maintenance

    ® ® CombiFlash Companion Installation Guide Section 9 Maintenance 9.1 Cleaning For general cleaning of the enclosure, use distilled water with a mild detergent. Use isopropyl alcohol for tougher stains. On printed areas, such as the rear panel, avoid rubbing vigorously or using aggressive solvents like acetone.

  • Page 72: Troubleshooting

    ® ® CombiFlash Companion XL Installation Guide 9.3 Troubleshooting 9.3.1 Electrical 1. If your instrument stops working and the front panel status LEDs (Power and Ready) are off, check the line cord connection. 2. If the line cord is connected properly, check the fuses in the power input module located on the Companion XL’s rear panel.

  • Page 73: Flow Cell Cleaning

    Section 9 Maintenance 9.4 Flow Cell Cleaning The Companion XL may alert you that flow cell cleaning is recommended. This alert message appears when flow cell light path is partially obstructed but the system is still operable. Cleaning the flow cell will keep the amount of noise and drift on the absorbance trace at a minimum and ensure accurate peak detection.
  • Page 74 ® ® CombiFlash Companion XL Installation Guide Table 9-1 Suggested Flow Cell Cleaning Solvents Solvent Description Methanol Miscible with most LC solvents. It will force other solvents through the system so that they will not interfere with the next sample. Breaks down the tertiary structure of silica and allows it to flush through.

  • Page 75: Alternative Cleaning Techniques

    Section 9 Maintenance 3. Pump 100 ml of solvent through the system at 100 mL/min. 4. If an alternative solvent was used, return the solvent inlet line to the appropriate solvent container. 5. Select the Tools>Auto Prime menu command to prime the system.
  • Page 76 ® ® CombiFlash Companion XL Installation Guide...
  • Page 77 Name and amount of Hazardous Substances or Elements in the product Hazardous Substances or Elements Component Name (Pb) (Hg) (Cd) (Cr(VI)) (PBB) (PBDE) Circuit Boards Name and amount of Hazardous Substances or Elements in the product O: Represent the concentration of the hazardous substance in this component’s any homogeneous pieces is lower than the ST/ standard limitation.
  • Page 79 Name and amount of Hazardous Substances or Elements in the product Hazardous Substances or Elements Component Name (Pb) (Hg) (Cd) (Cr(VI)) (PBB) (PBDE) LCD Display Circuit boards Wiring Internal Cables Line Cord Stepper Motor Deuterium lamp Valve Body Name and amount of Hazardous Substances or Elements in the product O: Represent the concentration of the hazardous substance in this component’s any homogeneous pieces is lower than the ST/ standard limitation.
  • Page 81 Mailing Address: P.O. Box 82531, Lincoln, NE 68501 Equipment Type/Environment: Laboratory Equipment for Light Industrial/Commercial Environments Trade Name/Model No: CombiFlash Companion XL with Touch screen and Foxy 200 Year of Issue: 2004 Standards to which Conformity is Declared: EN 61010-1...
  • Page 83 NE 68504, U.S.A. * This warranty applies to the USA and countries where Teledyne Isco Inc. does not have an authorized dealer. Customers in countries outside the USA, where Teledyne Isco has an authorized dealer, should contact their Teledyne Isco dealer for warranty service.

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