Prime The Solvent Lines - Teledyne CombiFlash Rf User Manual

4.10 Prime the Solvent Lines

The Rf 4x module was factory tested with 1:1 water/isopropyl
alcohol and subsequently purged with air. If residual fluids
would affect your chromatography, prime all channels on the
system.
Channel 1 should have been primed during the installation
steps in Section 2.14 (page 2-25). Channel 1 can be primed
using the Auto Prime menu command.
Channels 2 through 4 must be primed using the Manual
Control menu command. To do so:
1. Ensure that the solvent containers are filled.
2. Insert a priming tube assembly (from the accessory
package) between the upper and lower column mounts on
channels 2 through 4.
3. Select the "Tools>Manual Control" menu command.
4. Select the Column 2 the channel option.
5. Click the Prime B button. The system pumps 100% solvent
B through channel 2.
Note
When priming the first time, inspect the solvent and waste
connections to the system. If any leaks are observed, click the
Cancel button to stop the pump. Correct the leak by tightening the
fitting an additional
6. Allow the system to pump solvent B for at least 3 minutes.
Then, click the Cancel button.
7. Click the Prime A button. The system pumps 100% solvent
A through channel 2.
8. Allow the system to pump solvent A for at least 3 minutes.
Then, click the Cancel button.
Note
This B-A order leaves the internal lines filled with solvent
appropriate for the beginning of a purification run.
9. Repeat steps 4 through 8 for channels 3 and 4. Be sure to
change the channel option before starting the pumps.
Section 4 Preparation: Optional 4x Module
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1
turn, and then restart the prime.
4
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