Related Manuals for Teledyne 10
Summary of Contents for Teledyne 10
- Page 1 BioOptix™ 10 Installation and Operation Guide #69-5203-150 Copyright © 2004 Teledyne Isco, Inc. All rights reserved. Revision B, September 20, 2005...
- Page 3 Teledyne Isco recommends that you read this manual completely before placing the equipment in service. Although Teledyne Isco designs reliability into all equipment, there is always the possi- bility of a malfunction. This manual may help in diagnosing and repairing the malfunc- tion.
- Page 5 être affaiblie; cela augmentera votre risque de blessure. WARNING If this system uses flammable organic solvents, Teledyne Isco recommends that you place this system in a well-ventilated environment, designed for these types of materials.
- Page 6 AVERTISSEMENT Ce système peut utiliser des dissolvants organiques inflammables. Pour réduire le péril qui peut être causé par l'accumulation des vapeurs explosives, Teledyne Isco recommande que vous installez ce système dans un environnement bien-aéré qui est conçu pour les matières hasardeuses.
- Page 7 Hazard Symbols Warnings and Cautions Symboles de sécurité Warnungen und Vorsichtshinweise Advertencias y Precauciones The equipment and this manual use symbols used to warn of hazards. The symbols are explained below. Hazard Symbols The exclamation point within the triangle is a warning sign alerting you of important instructions in the instrument’s technical reference manual.
- Page 8 ™ BioOptix Safety...
-
Page 9: Table Of Contents
1.3 System Controls and Connectors ........1-3 1.3.1 BioOptix 10 Front Panel Controls ....... . 1-3 1.3.2 BioOptix 10 Left-side Controls and Connections . - Page 10 4.6 Service Department ........
- Page 11 1-3 BioOptix 10 right-side connections ........
- Page 12 1-4 BioOptix 10 Right-side Connections ........
-
Page 13: Section 1 Introduction
The purified sample is collected by a fraction collector — up to 300 fractions per sample for unattended operation, or unlimited fractions for attended purification runs. BioOptix 10 control software lets you quickly locate the fractions of interest. System highlights include: •... -
Page 14: System Specifications
Excludes Foxy 200 Fraction Collector and Windows PC Controller. Refer to the respective manuals for these additional specifications. b. Dry weight of the BioOptix 10, excluding connection cables, tubing, and user-supplied buffer containers. Operating voltage of the BioOptix 10 is factory set. -
Page 15: System Controls And Connectors
1.3 System Controls and Connectors 1.3.1 BioOptix 10 Front Panel Controls Figure 1-1 BioOptix 10 front panel Table 1-2 BioOptix 10 Front Panel Controls Item Name Manual Sample Inject Port Mode Knob Column Inlet Port Column Clamp Position Adjust Secures column clamp and allows for horizontal and rotational positioning of... -
Page 16: Biooptix 10 Left-Side Controls And Connections
BioOptix™ 10 Section 1 Introduction 1.3.2 BioOptix 10 Left-side Controls and Connections Figure 1-2 BioOptix 10 left-side connections Table 1-3 BioOptix 10 Left-side Connections Item Name Buffer A Inlet Ports Buffer B Inlet Ports Sample In Ports Pump Purge Port... -
Page 17: Biooptix 10 Right-Side Connections
BioOptix™ 10 Section 1 Introduction 1.3.3 BioOptix 10 Right-side Connections Figure 1-3 BioOptix 10 right-side connections Table 1-4 BioOptix 10 Right-side Connections Item Name Description Purified Sample Outlet Channels 1 through 10 which connect to the Foxy 200 Fraction Collector. -
Page 18: Biooptix 10 Rear Panel Controls And Connections
Section 1 Introduction 1.3.4 BioOptix 10 Rear Panel Controls and Connections Figure 1-4 BioOptix 10 rear panel controls and connections Table 1-5 BioOptix 10 Rear Panel Controls and Connections Item Name Cooling Fan External Control Connector Fuses AC Power Connector Description Ventilates the BioOptix 10 case and cools internal components. -
Page 19: Section 2 Installation
CAUTION Ensure there is at least 10 cm (4 inches) of air space on its left side so that the disconnect device (power switch) may be eas- ily accessed in an emergency. -
Page 20: Electrical Requirements
The containers may also be kept behind the unit as long as the con- tainers are accessible. The Foxy 200 should be installed to the right of the BioOptix 10. The fraction collector requires about 1800 square centimeters (275 in ) of bench space. -
Page 21: Accessing Pump And Column Module Internal Connections
1. Release the two sets of tubes from the retaining clips (Fig- ure 2-3). 2. Remove the four screws securing the back metal plate on the column module (Figure 2-3). Then, lift and remove the plate. Screws BioOptix™ 10 Section 2 Installation Column Module Screws Retaining Clips... -
Page 22: Routing The Sample Out Lines (Column Module)
BioOptix™ 10 Section 2 Installation Figure 2-4 Routing the Sample Out lines (column module) Figure 2-5 Pump controller connection 3. While facing the back of the column module, locate the two liquid tubing bundles secured to the lower-left inside case panel. -
Page 23: P110 Connection On Bd3
5. Connect the orange connector labeled BD3 P110 to P110 on the Power Drive Circuit Board (Figure 2-6). From Pump Module 6. Connect the plug labeled BD1 J106 to J106 on the Control- ler Circuit Board (Figure 2-7). From Pump Module BioOptix™ 10 Section 2 Installation... -
Page 24: Ac Power Connection From Pump Module
BioOptix™ 10 Section 2 Installation Figure 2-8 AC power connection from pump module Figure 2-9 Ribbon cable connections (top half of column module shown) 7. Plug the black power cord from the pump module into the receptacle at the lower part of the column module (Figure 2-8). -
Page 25: Liquid Connections
Figure 2-10 Liquid connections (pump module shown) 10. Connect the tubing from the column module to the pump module. Refer to Figure 2-10. a. Remove the two thumbscrews securing the splash guard to the pump module. Set aside the splash guard. -
Page 26: Aligning The Fasteners
BioOptix™ 10 Section 2 Installation Figure 2-11 Aligning the fasteners Figure 2-12 Column clamps inserted... -
Page 27: Assembling The Foxy 200
Kit. Connect the 25-pin connector labeled First Instrument Optix to the External Control port on the back of the Pump Module. The BioOptix 10 may now be positioned on the bench as shown in Figure 2-1. WARNING The system is heavy. Use a two-person lift to prevent injury. -
Page 28: Fluid Connections
Foxy to the RS-232-C port on the back of the Foxy 200. 7. Place the Foxy 200 to the right of the BioOptix 10 (Figure 2-1). Ensure that the full motion of the fraction collector arm is not obstructed, especially near column positions 9 and 10 on the BioOptix 10. -
Page 29: Buffer Assembly Routes A Common Solution To All Ten Channels
Cut to length a section of 0.250" OD Teflon tubing (P/N 023-0508-01) from the accessory kit and route the buffer solution to the common inlet. The standard length is 60" or 152 cm. BioOptix™ 10 Section 2 Installation Common Buffer Inlet Manifold... -
Page 30: Tubing Color Chart
023-0505-09) from the bulk roll in accessory kit. Foxy – The two bundles of tubing on the right side of the BioOptix 10 connect to the Foxy 200 Fraction Collector. Each color-coded tube is labeled with its channel number and finished with a threaded fitting. -
Page 31: Foxy 200 Tubing Connections
Figure 2-17 Adhesive retainers attached Refer to Figure 2-16 and complete the tubing connections between the BioOptix 10 and the Foxy 200. Thread and finger-tighten the fittings into the ten-channel drop former assembly. After connecting the tubing, peel the adhesive backing from each retainer. -
Page 32: Windows Pc Connection
RS-232 Serial Port on the PC. Install the BioOptix 10 Software on the Windows PC. 1. Locate the BioOptix 10 Software CD that was shipped with the system. 2. After turning on the PC, insert the CD into the CD-ROM drive. -
Page 33: Biooptix 10 System Preparation
1. Ensure that BioOptix 10 software is configured and run- ning on the connected Windows PC. 2. Turn the Power switch on the left side of the BioOptix 10 to 3. Press the Power button on the Foxy 200. 4. Place the Buffer A inlet line(s) into two liters of solution (such as HPLC-grade water or other appropriate buffer solution). -
Page 34: Column Bypass Tubing
Figure 2-18 Column bypass tubing Figure 2-19 Mode control knob 2-16 6. Ensure that each channel on the BioOptix 10 has 0.125" OD bypass tubing between the Column Inlet and Outlet ports (Figure 2-18). 7. For each channel, set the mode control knobs to Purge (Fig- ure 2-19). -
Page 35: Biooptix 10 Manual Hardware Control Window
9. In the Columns section of this window, check each channel number that should be primed. Configure the remaining settings to match those shown in Figure 2-20. 10. Click the Start Pump button. Observe the following for each enabled channel: Buffer B solution movement should be visible at the ❍... - Page 36 BioOptix™ 10 Section 2 Installation 2-18...
-
Page 37: Section 3 Operation
Note User-supplied clamps and apparatus may be used on the sys- tem. The mounting holes on the side of the BioOptix 10 accept 9.5 mm ( in) mounting stems. To install a column, insert the column into the clamp and turn the knob to close the clamp and secure the column. -
Page 38: Biooptix 10 Software
Note Refer to On-line Help for specific BioOptix 10 software operat- ing instructions. The operation of the BioOptix 10 system is directed by three files: • Gradient – these files define how a gradient is to be formed from Buffer A and B solutions. -
Page 39: Defining A Gradient
When creating a new method, the BioOptix 10 software con- figures the blocks of the method file according to a template file—standard.tpl. This template file can be modified to suit your applications, allowing up to ten blocks or tabs. -
Page 40: Equilibrate Settings
BioOptix™ 10 Section 3 Operation 3.5.1 Equilibrate Settings Figure 3-2 Equilibrate tab settings The default method file contains five blocks that are appropriate for use with affinity column purification: • Equilibrate – conditions the column. • Load – loads the sample onto the column. -
Page 41: Load Settings
• Injection – Select Pump to pump liquid sample from the Sample In ports onto the column. Select Manual to inject the sample from the manual injection ports (located on the top of the BioOptix 10) onto the column. Note that selecting Manual disables the flow rate and volume settings. -
Page 42: Wash Settings
BioOptix™ 10 Section 3 Operation 3.5.3 Wash Settings Figure 3-4 Wash tab settings 3.5.4 Elute Settings Figure 3-5 Elute tab settings This block flushes compounds that did not bind to the affinity media. The Wash tab is a fluid block type, therefore the settings displayed on this tab are the same as the Equilibrate tab. -
Page 43: Flush Settings
Many of the columns in the table are determined by the template file used to create the method file. (Refer to Appendix A for more details on the template file.) Default values will be assigned to the table cells from the method file you’ve chosen. BioOptix™ 10 Section 3 Operation... -
Page 44: Setting Up The Experiment
Windows-compatible file name. 2. Advance the cursor to the next cell to the right. BioOptix 10 completes the remaining cells in the row using the settings from the method file as defaults. 3. Repeat steps 1 and 2 for all channel positions that will be in use. -
Page 45: Saving The Experiment File
Save the experiment by selecting the File>Save menu command. Use a descriptive file name so that it can be located for later use. With the experiment file open, some BioOptix 10 operation buttons along the bottom of the window are active (Figure 3-8). -
Page 46: Operation Displays
This can be useful when comparing channels, especially when desiring to monitor the effects of any experimental interventions. The status bar is located at the bottom edge of the BioOptix 10 window. The fields in this bar display system information and messages during the run. -
Page 47: Post-Experiment Operation
At the completion of the experiment, the BioOptix 10 provides the option to flush the pumps (Figure 3-10). The pump flush will pass a wash fluid through the pumps to prevent salts from crys- tallizing in the system. -
Page 48: Saving Run Data
Buffer A and B mixing, and the stroke volume. Click the Save button to save your changes. The BioOptix 10 software automatically saves the run data and chromatograms using the experiment name as the filename. Two types of files are saved: “.lst”... -
Page 49: Autoreports
Section 3 Operation 3.8.3 AutoReports After the run the BioOptix 10 can automatically print the purifi- cation run results or save the file in a format listed in section 3.8.2. To automatically receive the hard copies or save the files, select the Tools>AutoReport menu command and modify the set-... - Page 50 BioOptix™ 10 Section 3 Operation 3-14...
-
Page 51: Section 4 System Maintenance
Flush the pumps daily to pre- vent damage. The BioOptix 10 system provides the option to flush the pump at the end of a purification run (see section 3.8). If the system will remain idle for a few hours or even overnight, a pump flush fol- lowing the run is recommended. -
Page 52: Exterior Cleaning
4.2 Exterior Cleaning 4.3 Piston Seal Replacement The BioOptix 10 system pumps the wash fluid, flushing the Buffer In lines, gradient formation components, pump, and purge lines. (See Figure B-6 in Appendix B.) For general cleaning of the enclosure, use distilled water with a mild detergent. -
Page 53: Access The Pump Assembly
Note Refer to Figure 4-3 for the following steps. 9. Remove the bottom retainer plate. 10. On the top of the pump assembly, remove the two 10-32 × " pan head screws and lock washers. 11. Lift the piston plug and top O-ring from the glass cylinder. -
Page 54: Pump Assembly Components
#6 × 1/4" Pan Head Screw Retainer Plate Figure 4-3 Pump assembly components Figure 4-4 Attach seal to push rod 10-32 × 3/8" Pan Head Screw #10 Interior Lock Washer #10 Flat Washer Inductor #8-32 × 1-1/4" Pan Head Screw... -
Page 55: Deuterium Lamp Replacement
18. Refer to Figure 4-3 and reassemble the pump channel using new O-rings at the top and bottom of the glass cylin- der. 19. Repeat steps 10 through 18 for the remaining channels. Note Channel six is located below the inductor. To remove the glass cylinder on this channel, first remove the inductor by unfasten- ing the #8 pan head screw and internal lock washer. -
Page 56: Waste Port Mounting Plate
BioOptix™ 10 Section 4 System Maintenance Figure 4-5 Waste port mounting plate Lamp Mounting Screws Twist Lock Clamp Figure 4-6 Deuterium lamp location CAUTION Hot surfaces may cause burns. The UV lamp and nearby sur- faces are hot. To remove the lamp: 1. -
Page 57: Troubleshooting
Prime all channels to overcome the added minute leakage in each channel. Make sure the bypass tubes are in place on all channels. Inlet lines clogged; insufficient filtering. Inlet lines are not securely connected. Air leakage at internal fitting. BioOptix™ 10 Section 4 System Maintenance... -
Page 58: Leaks
1. If your instrument stops working, check the line cord con- nection. 2. If the line cord is connected properly, check the fuses in the power input module located on the BioOptix 10 rear panel. These are time delay fuses (T-type) and rated according to the mains input power voltage: 100 to 129 VAC mains input: 2.0 A fuse... -
Page 59: Circuit Breaker Location
The thermal breaker is located behind the waste port panel (Figure 4-6). To reset the breaker, open the panel and locate the reset switch (Figure 4-8). Insert a small flat blade screwdriver into the opening and push the switch in to the right. BioOptix™ 10 Section 4 System Maintenance... -
Page 60: Service Department
The warranty at the end of the manual describes the conditions under which Teledyne Isco will pay surface shipping costs. It is very important that the unit be well-packaged for shipping and fully insured. -
Page 61: Appendix A Method Templates
Appendix A Method Templates A.1 Opening the Template When creating a new method, the BioOptix 10 software con- figures the blocks of the method file according to a template file. This template file can be modified, allowing it to be customized for many applications. -
Page 62: A-1 Installed Standard.tpl File
3. Change the Files of type pull-down list to All files. 4. Use the Open dialog box to locate the Standard.tpl file. Default installations of BioOptix 10 software place this in the Program Files folder on the hard drive. Typically the path is C:\Program Files\BioOptix 10\Standard.tpl. -
Page 63: Modifying The Template
Figure A-2 is an example of a comment. BioOptix™ 10 Appendix A Method Templates Block Count = total number of blocks (value range: 1 to 10) Name = Descriptive block title (length: 0 to 20 characters) Type = Block function... -
Page 64: Blocks
End of the block. If this block in the method should allow a choice to pause the operation, enter Yes. Enter No to disable the choice. When disabled, the BioOptix 10 automatically proceeds to the next block. This section contains the UV detection wavelength setting. This value assigns a default wavelength for the method. -
Page 65: Appendix B Fluid Path Diagrams
Figure B-1 Fluid path for Fluid-type blocks This appendix contains simplified diagrams of the plumbing and valves internal to the BioOptix 10 system. Various operating states are depicted for a single channel. A complete plumbing diagram can be found in Figure B-7. -
Page 66: Load-Type Blocks
BioOptix™ 10 Appendix B Fluid Path Diagrams Figure B-2 Inactive channel B.2 Load-type Blocks Figure B-3 Fluid path for Load-type blocks - Pump injections While the system is running a fluid-type block: • The Buffer valve switches between buffers A and B to produce the correct mix. -
Page 67: Gradient-Type Blocks
Mode valve to the Manual Inject position. The liquid sample is then forced through the injection port on top of the BioOptix 10 system. The liquid sample is intro- duced directly onto the column (Figure B-4). -
Page 68: Pump Flush
BioOptix™ 10 Appendix B Fluid Path Diagrams B.4 Pump Flush Figure B-6 Pump flush B.5 Plumbing Diagram A Pump Flush should be done whenever the system will be idle for more than a few hours. (See sections 3.8 and 4.1.) The flush removes salts from the Buffer in lines to the Mode knob (Figure B-6). -
Page 69: B-7 Biooptix 10 Plumbing Diagram
BioOptix™ 10 Appendix B Fluid Path Diagrams Figure B-7 BioOptix 10 Plumbing Diagram... - Page 70 BioOptix™ 10 Appendix B Fluid Path Diagrams...
-
Page 71: Appendix C Replacement Parts Lists
Replacement parts are called out in illustrations in this section. Reference the call-outs in the accompanying tables to determine the part number for the item. Replacement parts can be purchased by contacting Teledyne Isco’s Customer Service Department. Teledyne Isco, Inc. - Page 72 BioOptix™ 10 Appendix C Replacement Parts Lists...
-
Page 73: Detector Assembly
13BioOptix™ 10 Replacement Parts Lists 13BioOptix™ 10 Replacement Parts Lists C.1 Detector Assembly... - Page 74 13BioOptix™ 10 Replacement Parts Lists...
- Page 75 13BioOptix™ 10 Replacement Parts Lists...
- Page 76 13BioOptix™ 10 Replacement Parts Lists...
-
Page 77: Column Module
13BioOptix™ 10 Replacement Parts Lists C.2 Column Module... - Page 78 13BioOptix™ 10 Replacement Parts Lists...
- Page 79 13BioOptix™ 10 Replacement Parts Lists...
- Page 80 13BioOptix™ 10 Replacement Parts Lists C-10...
-
Page 81: Pump Module
13BioOptix™ 10 Replacement Parts Lists C.3 Pump Module C-11... - Page 82 13BioOptix™ 10 Replacement Parts Lists C-12...
- Page 83 13BioOptix™ 10 Replacement Parts Lists C-13...
- Page 84 13BioOptix™ 10 Replacement Parts Lists C-14...
-
Page 85: Declaration Of Conformity
Teledyne Isco, Inc. 4700 Superior, Lincoln, Nebraska 68504 USA Mailing Address: P.O. Box 82531, Lincoln, NE 68501 Laboratory Equipment for Light Industrial/Commercial Environments Bio Optix 10 System 2005 EN 61326-1998 EMC Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use... - Page 87 * This warranty applies to the USA and countries where Teledyne Isco Inc. does not have an authorized dealer. Customers in countries outside the USA, where Teledyne Isco has an authorized dealer, should contact their Teledyne Isco dealer for warranty service.