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have not yet prepared the sample. You must also
specify the cartridge type and size (Column plan
subscribers only). When you click the run button, the
system will perform a column equilibration and then
wait while you prepare the cartridge. After you have
placed the cartridge on the system, click OK to
continue with the run.
• Solid – Select this option if you have prepared the
sample and placed it into a solid sample load
cartridge. You must also specify the cartridge type
and size (Column plan subscribers only). The system
will proceed automatically until the end of the
programmed run length.
• Liquid – Select this option if you have prepared a
liquid sample and plan to manually inject it into the
injection port after column equilibration.
• None (on column) – Select this option if you have
pre-loaded the sample on the column. The system will
skip column equilibration so that the sample will not
be flushed from the column before the run.
Loading Type options for the Rf 150 system are:
• Equilibration – Select this option if you intend to
equilibrate the column before introducing the liquid
or solid sample.
• No Equilibration – Select this option to skip column
equilibration.
5. Review or update the Start Rack and Start Tube.
6. Review the Minimum Run Requirements. The system will
report the estimated solvent volumes, expected waste,
collection tube usage, etc. You can use this information to
verify that there will be enough solvent to complete the
run, the waste volume does not exceed the collection
container's capacity, and whether or not more tubes will be
required during the run.
Note
+
The Rf
systems, including those equipped with 4x, estimate the
solvent volumes by monitoring the solvent level in the container
and the known usage rate. The systems continue to refine this
Section 5 Operation
5-7
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