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CombiFlash® Rf
User Manual
from about 0.2 to 2 times the peak width setting. For
example, if you entered a peak width of 1 minute, the
range would be 12 seconds to 2 minutes. For best
operation, the peak width should be set to just over
the average peak width being separated. For
instance, if the average peak width is 45 seconds, you
should enter a peak width of 1 minute. For most flash
chromatography, 1 minute is a good starting point for
a peak width.
• Threshold Detection — Select this option to enable
Threshold peak detection. When enabled, peaks will
be recorded if the Absorbance Units (AU) value is
exceeded.
• Threshold — Type or select the Absorbance Units
value to be used for Threshold detection.
Note: If both Slope and Threshold peak detection
methods are checked, the system considers a peak to
be present when any one condition is met. This logical
OR operator means that the system will cut a peak
when either the Slope condition is true, or when the
Threshold condition is true.
• Monitor — Enable this option to only use the
detection source as a monitor. When enabled, the
detection source will be displayed as a trace on the
gradient plot area, but will not be used to cut peaks.
• All Wavelength Detection — Enable this option to
detect peaks within a user-selected range of
wavelengths. When enabled, click the Details button to
configure additional settings. These settings include the
slope-based and peak width options described in 1 and
2 above. Additionally, you can type or select the
minimum and maximum wavelengths limits in
nanometers. Peak Width for All Wavelength Detection
also determines the length of time before an automatic
auto-zero of baseline occurs. This period is twice the
peak width setting.
• External Detector — (Rf+ systems, including those
equipped with 4x) Enable this option to use a 0 to 1 volt
analog input signal from an external detector. When
enabled, click the Details button to configure additional
settings. These settings include the slope-based and
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